Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
USF-2 CRISPR/Cas9 KO Plasmid (m): sc-423629
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- USF-2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the USF-2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: USF-2 Antibody (5E9): sc-293443
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
USF-2 CRISPR/Cas9 KO Plasmid (m) | sc-423629 | 20 µg | $397.00 |
Overview
Upstream transcription factor 2 (USF-2), encoded by the mouse Usf2 gene, is a basic helix–loop–helix leucine zipper transcription factor that binds E-box motifs to regulate promoter activity across metabolic and stress-responsive gene networks. USF-2 participates in transcriptional control of glucose and lipid metabolism, cell-cycle progression, and differentiation programs, and it can modulate chromatin context through interactions with co-regulators. In murine systems, altered USF-2 activity has been linked to dysregulated metabolic homeostasis and changes in proliferative signaling, supporting its relevance to studies of cardiometabolic and cancer-associated pathways. Usf2 is therefore frequently investigated as a node connecting nutrient sensing, transcriptional circuitry, and tissue-specific gene expression.
USF-2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Usf2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Usf2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Usf2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish USF-2 protein expression.
This CRISPR knockout system enables efficient generation of Usf2-deficient cell models for investigation of USF-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Usf2 exon(s) critical for USF-2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Usf2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by USF-2 CRISPR/Cas9 KO Plasmid (m) and USF-2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Usf2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by USF-2 HDR Plasmid (m) and USF-2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Usf2 homology arms to support homology-directed repair at defined Usf2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.