Date published: 2026-6-5
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CRISPR/dCas9 Activation Plasmids consist of three plasmids at a 1:1:1...
CRISPR/dCas9 Activation Plasmids consist of three plasmids at a 1:1:1 mass ratio. The second plasmid encodes the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene.
CRISPR/dCas9 Activation Plasmids consist of three plasmids at a 1:1:1 mass ratio. The second plasmid encodes the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene.

Cre Vector nocaute CRISPR Knockout e Produtos de Ativação (): sc-418923

4.5(2)
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Fichas de dados
  • 20 µg de ADN plasmático purificado, pronto para transfecção; Adequado para até 20 transfecções
  • O vetor Cre expressa a recombinase Cre para catalisar a recombinação de ADN específica do local entre dois locais LoxP
  • O vetor Cre foi concebido para visar especificamente os sítios LoxP que ladeiam os marcadores de seleção inseridos pelo plasmídeo HDR e excisar o material genético
  • O vetor Cre contém um promotor CMV para conduzir a expressão da recombinase Cre
  • Após a transfecção, as células podem ser analisadas por WB, IF ou IHC utilizando anticorpos para o gene alvo, ou re-transfectadas para edição adicional de ADN
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Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

Vetor Cre

sc-418923
20 µg
$83.00

do you have any educational information

Asked by: Gary
For assistance please contact Technical service . Please call 800-457-3801 or email scbt@scbt.com.
Answered by: BlakeJ
Date published: 2026-01-21

Hi, can the above plasmid be used for yeast namely saccharomyces cerevisiae?

Asked by: NT777
.
Answered by: lai13
Date published: 2024-07-25

How to transfect the cre-vector to the cells that already have puromycin gene incorporated by HDR vector? transfect, then wait two or three days and consider those cells puromycin free? correct? do I select?

Asked by: michaela
.
Answered by: lai13
Date published: 2024-07-25

Can you provide the plasmid map and size of the vector? Thanks,

Asked by: Chris1
.
Answered by: lai13
Date published: 2024-07-25

Does this kit includes a vector to flanked my target gene?

Asked by: Ldas01
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2022-01-26

How large is this plasmid?

Asked by: Dkr0
Thank you for your question. Our Cre Vector sc-418923 has a size of approximately 5.9 kilobases.
Answered by: Tech Support Europe
Date published: 2024-04-20

Why is CRE Vector Transfection "Recommended" after for CRISPR/Cas9 KO Plasmid and HDR Plasmid Transfection (following selection)? Is it simply to remove the selection marker? Or, does it help in any way with knocking out the gene?

Asked by: jmd6207
Thank you for your question. It is not mandatory, and only recommended if you wish to removed the puromycin selection marker. Removing the HDR plasmid insert using the CRE vector does not help with knocking out the gene. It is useful if you wish you knock out a second gene or if you need you cells to no longer be puromycin resistant.
Answered by: Tech Service
Date published: 2024-03-19

whats the cre loxp systems

Asked by: waleed xel
Thank you for your question. The Cre-Lox system is a site-specific recombinase technology, used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. You can find our more about the Cre-Lox system at any of the links below: https://en.wikipedia.org/wiki/Cre-Lox_recombination https://www.youtube.com/watch?v=oLPjiwM0G7A&app=desktop
Answered by: Tech Service
Date published: 2022-05-04
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Rated 5 out of 5 by from Very useful product.Very useful tool to achieve double or triple knock-out with the CRISPR system since the most of Santa Cruz CRISPR products are resistant to the same antibiotic-pyromycin.
Date published: 2017-04-25
Rated 4 out of 5 by from Remove the marker gene efficientlyI used this vector to remove the neo marker-gene in the pig primary cells . The marker-free efficiency was so high to 80% .I nucleofected the 4ug Cre vector to the 1000000 cells by ALONZO. But, i suggested that can the CAG promoter may replace the CMV promoter of this vector, because of the CMV promoter may Cause gene-silencing in some special cell lines
Date published: 2016-11-21
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Cre Vector nocaute CRISPR Knockout e Produtos de Ativação () is rated 4.5 out of 5 by 2.
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