SYP/Synaptophysin Antibody (A-9): sc-365488

SYP Antibody (A-9) is a mouse monoclonal IgG2b antibody that detects synaptophysin (SYP) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). SYP is an integral membrane protein predominantly found in neurons and endocrine cells, playing a crucial role in the regulation of synaptic vesicle dynamics. SYP forms hexameric complexes that contribute to the formation of large conductance channels, which are essential for neurotransmitter release and synaptic transmission. SYP contains four transmembrane domains and is N-glycosylated, which is important for stability and function. SYP interacts with membrane cholesterol, facilitating vesicle formation and recycling, a process vital for maintaining synaptic function. SYP forms complexes with synaptobrevin (VAMP), which are upregulated during neuronal development, although these complexes are not essential for the exocytosis process itself. These complexes serve as a reservoir of synaptobrevin, ensuring a readily available supply for neurotransmitter release. Dynamic interactions of SYP with other proteins, such as brain Myosin V, further underscore SYP′s importance in synaptic physiology, particularly during calcium-mediated signaling events that trigger neurotransmitter release.

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SYP/Synaptophysin Antibody (A-9) References:

1. Inhibition of catechol-O-methyl transferase (COMT) by tolcapone restores reductions in microtubule-associated protein 2 (MAP2) and synaptophysin (SYP) following exposure of neuronal cells to neurotropic HIV.  |  Lee, TT., et al. 2015. J Neurovirol. 21: 535-43. PMID: 26037113
2. Lentiviral-Mediated Netrin-1 Overexpression Improves Motor and Sensory Functions in SCT Rats Associated with SYP and GAP-43 Expressions.  |  Han, XF., et al. 2017. Mol Neurobiol. 54: 1684-1697. PMID: 26873853
3. Synaptophysin Is a Reliable Marker for Axonal Damage.  |  Gudi, V., et al. 2017. J Neuropathol Exp Neurol. 76: 109-125. PMID: 28177496
4. Synthesis and biological activity of benzoylcyclohexanedione herbicide SYP-9121.  |  Li, H., et al. 2017. Pestic Biochem Physiol. 142: 155-160. PMID: 29107240
5. Aberrant DNA methylation of synaptophysin is involved in adrenal cortisol-producing adenoma.  |  Zhong, JY., et al. 2019. Aging (Albany NY). 11: 5232-5245. PMID: 31352437
6. Sexual experience in female mice involves synaptophysin-related plasticity in the accessory olfactory bulb.  |  Marco-Manclus, P., et al. 2022. Physiol Behav. 244: 113649. PMID: 34798129
7. Epigenetic alternations in the SYP and DLG4 genes due to low-level methylmercury exposure during neuronal differentiation in vitro.  |  Kurita, H., et al. 2024. J Appl Toxicol. 44: 1986-1996. PMID: 39187442
8. The role of Imp and Syp RNA-binding proteins in precise neuronal elimination by apoptosis through the regulation of transcription factors.  |  Guan, W., et al. 2024. Elife. 12: PMID: 39364747
9. Receptor-binding, tyrosine phosphorylation and chromosome localization of the mouse SH2-containing phosphotyrosine phosphatase Syp.  |  Feng, GS., et al. 1994. Oncogene. 9: 1545-50. PMID: 8183548
10. Calcium-dependent serine phosphorylation of synaptophysin.  |  Rubenstein, JL., et al. 1993. Synapse. 13: 161-72. PMID: 8383357

The preferred SYP/Synaptophysin antibody is SYP/Synaptophysin Antibody (D-4): sc-17750.