PABP Antibody (A-4): sc-166381

PABP Antibody (A-4) is a mouse monoclonal IgG3 kappa light chain antibody that detects PABP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PABP Antibody (A-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Poly(A)-binding protein (PABP) plays a crucial role in the regulation of mRNA metabolism, including translation initiation, stability, and degradation. Located predominantly in the cytoplasm, PABP interacts with the poly(A) tail of eukaryotic mRNA, which is vital for the recruitment of translation initiation factors and the formation of the translation initiation complex. This interaction not only enhances the efficiency of translation but also protects mRNA from degradation, thereby influencing gene expression levels. The modular structure of PABP, featuring four N-terminal RNA-binding domains and a large C-terminal domain, allows for multiple protein-protein interactions, including with eukaryotic translation initiation factor 4G (eIF4G), which is essential for bridging the mRNA ends and facilitating the assembly of the translation machinery. The human PABP gene is located on chromosome 8q22.2-q23 and encodes a protein consisting of 633 amino acids, underscoring PABP′s importance in cellular processes and potential implications in viral infections, where PABP cleavage can lead to host translation shutoff.

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PABP Antibody (A-4) References:

1. Poly(A) binding protein (PABP) homeostasis is mediated by the stability of its inhibitor, Paip2.  |  Yoshida, M., et al. 2006. EMBO J. 25: 1934-44. PMID: 16601676
2. Stimulation of picornavirus replication by the poly(A) tail in a cell-free extract is largely independent of the poly(A) binding protein (PABP).  |  Svitkin, YV., et al. 2007. RNA. 13: 2330-40. PMID: 17942745
3. Pabp binds to the osk 3'UTR and specifically contributes to osk mRNA stability and oocyte accumulation.  |  Vazquez-Pianzola, P., et al. 2011. Dev Biol. 357: 404-18. PMID: 21782810
4. PABP is not essential for microRNA-mediated translational repression and deadenylation in vitro.  |  Fukaya, T. and Tomari, Y. 2011. EMBO J. 30: 4998-5009. PMID: 22117217
5. PABP and the poly(A) tail augment microRNA repression by facilitated miRISC binding.  |  Moretti, F., et al. 2012. Nat Struct Mol Biol. 19: 603-8. PMID: 22635249
6. Studies on human eRF3-PABP interaction reveal the influence of eRF3a N-terminal glycin repeat on eRF3-PABP binding affinity and the lower affinity of eRF3a 12-GGC allele involved in cancer susceptibility.  |  Jerbi, S., et al. 2016. RNA Biol. 13: 306-15. PMID: 26818177
7. Viral and cellular mRNA-specific activators harness PABP and eIF4G to promote translation initiation downstream of cap binding.  |  Smith, RWP., et al. 2017. Proc Natl Acad Sci U S A. 114: 6310-6315. PMID: 28559344
8. PABP Cooperates with the CCR4-NOT Complex to Promote mRNA Deadenylation and Block Precocious Decay.  |  Yi, H., et al. 2018. Mol Cell. 70: 1081-1088.e5. PMID: 29932901
9. Tailing Off: PABP and CNOT Generate Cycles of mRNA Deadenylation.  |  Bresson, S. and Tollervey, D. 2018. Mol Cell. 70: 987-988. PMID: 29932908
10. UBR-5 and UBE2D mediate timely exit from stem fate via destabilization of poly(A)-binding protein PABP-2 in cell state transition.  |  Calva Moreno, JF., et al. 2024. Proc Natl Acad Sci U S A. 121: e2407561121. PMID: 39405353

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PABP Antibody (A-4) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-PABP Antibody (A-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PABP Antibody (A-4) Specifications

Alternate Product Name: PABP

Product Clone Name: A-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 11-37

Epitope Target: PABP

Epitope Species: human

Product Reactivity/Detection: PABP of mouse, rat and human origin, PABPC1L, PABPC3 and PABPC4 of human origin, PABPC2 and PABPC6 of mouse origin and the corresponding rat homologs

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for PABP Antibody (A-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred PABP antibody is PABP Antibody (10E10): sc-32318.