Nup153 CRISPR/Cas9 KO Plasmid (h): sc-403206
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nup153 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Nup153 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nup153 Antibody (R3G1): sc-101544
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nup153 CRISPR/Cas9 KO Plasmid (h) | sc-403206 | 20 µg | $397.00 |
Overview
NUP153 encodes Nup153, a scaffold nucleoporin of the nuclear pore complex that contributes to nuclear basket architecture and regulates nucleocytoplasmic transport. Nup153 participates in nuclear pore assembly and disassembly during the cell cycle, interfaces with importin/exportin pathways, and helps coordinate chromatin organization and transcriptional regulation at the nuclear periphery. Through these roles, Nup153 influences RNA processing and genome maintenance by controlling nuclear access of regulatory factors. Altered nucleoporin function, including disruption of NUP153-associated transport and nuclear envelope dynamics, has been linked to mechanisms relevant to tumor biology and neurodevelopmental phenotypes in cellular and genetic studies.
Nup153 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NUP153 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NUP153 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NUP153 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Nup153 protein expression.
This CRISPR knockout system enables efficient generation of NUP153-deficient cell models for investigation of Nup153 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NUP153 exon(s) critical for Nup153 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NUP153 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Nup153 CRISPR/Cas9 KO Plasmid (h) and Nup153 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NUP153 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Nup153 HDR Plasmid (h) and Nup153 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NUP153 homology arms to support homology-directed repair at defined NUP153 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.