Hrs Antibody (D-11): sc-271160

Hrs Antibody (D-11) is a mouse monoclonal IgG1 antibody that detects hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Hrs is a crucial zinc-finger protein that plays a significant role in intracellular trafficking and signal transduction, particularly associated with early endosomes. Hrs (D-11) antibody recognizes a unique structure including a phosphatidylinositol 3-phosphate-binding domain, which is vital for endosomal targeting, allowing Hrs to co-localize with Clathrin through a Clathrin box motif located at its carboxy-terminus. This interaction is essential for the proper functioning of endosomal pathways, which are critical for cellular communication and nutrient uptake. Additionally, Hrs is involved in ventral folding morphogenesis and shares structural similarities with the yeast protein Vps27p, known for its role in vacuolar protein sorting. The human Hrs gene, located on chromosome 17q25, encodes a 777 amino acid protein that is integral to various cellular processes. Notably, in Schwann cells, Hrs co-localizes with the tumor suppressor protein schwannomin at endosomes, indicating a potential collaborative role in Hrs-mediated cell signaling pathways. Anti-Hrs antibody (D-11) is an invaluable tool for researchers studying these complex mechanisms in various biological contexts.

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Hrs Antibody (D-11) References:

1. The neurofibromatosis 2 tumor suppressor protein interacts with hepatocyte growth factor-regulated tyrosine kinase substrate.  |  Scoles, DR., et al. 2000. Hum Mol Genet. 9: 1567-74. PMID: 10861283
2. Endosomal localization and receptor dynamics determine tyrosine phosphorylation of hepatocyte growth factor-regulated tyrosine kinase substrate.  |  Urbé, S., et al. 2000. Mol Cell Biol. 20: 7685-92. PMID: 11003664
3. The NF2 interactor, hepatocyte growth factor-regulated tyrosine kinase substrate (HRS), associates with merlin in the 'open' conformation and suppresses cell growth and motility.  |  Gutmann, DH., et al. 2001. Hum Mol Genet. 10: 825-34. PMID: 11285248
4. Huntingtin-associated protein 1 interacts with hepatocyte growth factor-regulated tyrosine kinase substrate and functions in endosomal trafficking.  |  Li, Y., et al. 2002. J Biol Chem. 277: 28212-21. PMID: 12021262
5. Functional analysis of the relationship between the neurofibromatosis 2 tumor suppressor and its binding partner, hepatocyte growth factor-regulated tyrosine kinase substrate.  |  Sun, CX., et al. 2002. Hum Mol Genet. 11: 3167-78. PMID: 12444101
6. The ESCRT-0 component HRS is required for HIV-1 Vpu-mediated BST-2/tetherin down-regulation.  |  Janvier, K., et al. 2011. PLoS Pathog. 7: e1001265. PMID: 21304933
7. ESCRT-0 Component Hrs Promotes Macropinocytosis of Kaposi's Sarcoma-Associated Herpesvirus in Human Dermal Microvascular Endothelial Cells.  |  Veettil, MV., et al. 2016. J Virol. 90: 3860-3872. PMID: 26819309
8. ErbB3 interacts with Hrs and is sorted to lysosomes for degradation.  |  Fosdahl, AM., et al. 2017. Biochim Biophys Acta Mol Cell Res. 1864: 2241-2252. PMID: 28867611
9. The ESCRT-0 subcomplex component Hrs/Hgs is a master regulator of myogenesis via modulation of signaling and degradation pathways.  |  Coudert, L., et al. 2021. BMC Biol. 19: 153. PMID: 34330273
10. Axonal transport of Hrs is activity dependent and facilitates synaptic vesicle protein degradation.  |  Birdsall, V., et al. 2022. Life Sci Alliance. 5: PMID: 35636965

The preferred Hrs antibody is Hrs Antibody (C-7): sc-271455.