G9a抗体(C-9): sc-515726

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- 反-G9a抗体 C-9はマウスモノクローナルIgG₁G9a 抗体です。200 µg/mlで提供
- human由来のG9aの内部領域に位置するアミノ酸raised against amino acids 346-564 mapping within an internal region of G9a of human originに対応
- 反-G9a抗体 (C-9) mouse, rat と human 由来のG9a WB, IP, IF と ELISAでの検出にはお勧めします
- 反-G9a抗体 (C-9) IP用のアガロース、に共役での利用可能です。WB、IHC(P)とELISA用のHRP、IF、IHC(P)とFCM用のphycoerythrin または FITC、に共役での利用可能です。
- WB (RGB)、IF、IHC(P)、FCM、iBright™ FL1000、FluorChem™、Typhoon、Azureと他の同等システムでRGB蛍光イメージングシステム用のAlexa Fluor^® 488、Alexa Fluor^® 546、Alexa Fluor^® 594 または Alexa Fluor^® 647、に共役での利用可能です。
- WB (NIR)、IF、FCMとLI-COR^®/Odyssey^®、iBright™ FL1000、FluorChem™、Typhoon、Azureと他の同等システムで近赤外(NIR)検出法用のAlexa Fluor^® 680 または Alexa Fluor^® 790、に共役での利用可能です。
- G9a (C-9): sc-515726の無料の10 µgサンプルを受け取るには、ご連絡ください。
- m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for G9a Antibody (C-9) for WB applications. This reagent is now offered in a bundle with G9a Antibody (C-9) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

関連項目

G9a細胞応答の機能解析のためのChemCruz™ Chemical G9a 阻害剤

G9a (C-9): sc-515726. Western blot analysis of G9a expression in...

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G9a Antibody (C-9) is a high quality monoclonal G9a antibody (also designated EHMT2 antibody, Euchromatic Histone Lysine Methyltransferase 2 antibody, KMT1C antibody, Euchromatic Histone-Lysine N-Methyltransferase 2 antibody, Histone-Lysine N-Methyltransferase EHMT2 antibody, Histone H3-K9 Methyltransferase 3 antibody, HLA-B Associated Transcript 8 antibody, Lysine N-Methyltransferase 1C antibody, H3-K9-HMTase 3 antibody, C6orf30 antibody, BAT8 antibody, NG36 antibody, Chromosome 6 Open Reading Frame 30 antibody, or G9A Histone Methyltransferase antibody) suitable for the detection of the G9a protein of mouse, rat and human origin. G9a Antibody (C-9) is available as both the non-conjugated anti-G9a antibody form, as well as multiple conjugated forms of anti-G9a antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Distinct modifications of histone tails, such as acetylation, phosphorlation and methylation, regulate nuclear processes, such as control of transcription and mitotic chromosome condensation. Histone methyltransferases (HMTases) are among the different groups of enzymes known to catalyze the covalent modification. G9a, a SET domain-containing protein, is a novel mammalian lysine-preferring HMTase. G9a, also known as BAT8, NG36 or HMTase (for mammalian histone methyltransferase), has strong HMTase activity towards histone H3 lysine 9 methylation in vitro (2-4). G9a plays a dominant role in euchromatic histone H3 lysine 9 methylation, is essential for early embryogenesis and is involved in the transcriptional repression of developmental genes. Like SUV39H, G9a transfers methyl groups to the lysine residues of histone H3, but with a 10-20-fold higher activity than SUV39H1. G9a also adds methyl groups to lysine 27 as well as lysine 9 in histone H3. G9a localizes in the nucleus, indicating that it may contribute to the organization of the higher order chromatin structure of non-centromeric loci. The human G9a gene maps to chromosome 6p21.3 (3,5,6).

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

動物種	遺伝子名	Gene ID	Chromosome Location	Isoform (mRNA) Accession #	Protein Accession #	OMIM™ Number
Human	EHMT2	10919	6p21.33	XM_005248824, XM_006714974, XM_006714975, XM_006714976, XM_017010202, NM_001289413, NM_001318833, NM_006709, NM_025256	Q96KQ7	604599
Mouse	Ehmt2	110147	17 B1	XM_006523482, XM_011246248, XM_011246249, NM_001286573, NM_001286575, NM_145830, NM_147151	Q9Z148	N/A

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I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with G9a (C-9): sc-515726?

質問者: cjMara

We recommend using one of our our exclusive Mouse IgG Binding Proteins conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety

回答者: Technical Support

投稿日: 2017-02-27

Minkaによって、 5つ星のうち 5 と評価されています。 exellent on mouse cell lysates Cell lysates of mouse macrophages , works well in 1: 250 dilution

投稿日: 2018-02-01

shinyhによって、 5つ星のうち 5 と評価されています。 Great Band!!! This antibody is very nice. i got the nice band!!!

投稿日: 2017-08-31

xiangbingによって、 5つ星のうち 5 と評価されています。 Great features! We used the sample antibody and got expected result by western blotting.

投稿日: 2017-08-10

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G9a抗体(C-9): sc-515726

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関連項目

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with G9a (C-9): sc-515726?

G9a抗体(C-9): sc-515726

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関連項目

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with G9a (C-9): sc-515726?

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