Date published: 2025-9-9

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CD81抗体(5A6): sc-23962

4.1(14)
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データシート
  • CD81抗体 (5A6)はマウスモノクローナルIgG1 κです。200 µg/mlで提供
  • 卵白アルブミン結合体合成ペプチドRKRVPDHHPCに対する
  • 反-CD81抗体 (5A6) mouse, rat と human 由来のCD81 WB, IP, IF と FCMでの検出にはお勧めします
  • 抗 CD81 抗体 (5A6) は、IP 用には アガロース、WB、IHC(P)、ELISA 用には HRP、IF、IHC(P)、FCM 用には フィコエリスリン または FITC にそれぞれ結合したものが利用可能
  • WB (RGB)、IF、IHC(P)、FCM、iBright™ FL1000、FluorChem™、Typhoon、Azureと他の同等システムでRGB蛍光イメージングシステム用のAlexa Fluor® 488Alexa Fluor® 546Alexa Fluor® 594 または Alexa Fluor® 647、に共役での利用可能です。
  • WB (NIR)、IF、FCMとLI-COR®/Odyssey®、iBright™ FL1000、FluorChem™、Typhoon、Azureと他の同等システムで近赤外(NIR)検出法用のAlexa Fluor® 680 または Alexa Fluor® 790、に共役での利用可能です。
  • CD81 (5A6): sc-23962無料の10 µgサンプルを受け取るには、ご連絡ください。
  • m-IgG Fc BP-HRPおよび 1 BP-HRP">m-IgG1 BP-HRPは、CD81 Antibody (5A6) for WB applications. の二次検出試薬として推奨されています。これらの試薬は現在、CD81 Antibody (5A6) とバンドルして提供されています(下記の注文情報を参照)。

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関連項目

CD81抗体 (5A6) は、CD81タンパク質を標的として特別に設計されたマウスモノクローナルIgG1κ軽鎖抗体です。CD81は、4つの膜貫通ドメインによって特徴づけられるテトラスパニンファミリーの主要メンバーであり、TAPA-1としても知られています。CD81は、造血系、神経外胚葉系、間葉系の細胞に広く発現しており、細胞増殖、シグナル伝達、および膜微小ドメインの形成において重要な役割を果たしています。CD81は、パルミトイル化や糖鎖付加などの翻訳後修飾を必要とし、これらは適切な局在、安定性、およびCD37、CD53、CD19、CD21、MHCクラスII抗原などの他の膜タンパク質との相互作用に不可欠です。抗CD81抗体(5A6)は、OCI-LY8 Bリンパ腫細胞株を用いて開発され、ウェスタンブロッティング(WB)、免疫沈降(IP)、免疫蛍光(IF)、フローサイトメトリー(FCM)などの手法により、マウス、ラット、ヒトのCD81を効果的に検出することができます。西洋ワサビペルオキシダーゼ(HRP)、フィコエリトリン(PE)、フルオレセインイソチオシアネート(FITC)、および各種Alexa Fluor® 結合体などの結合体、および非結合体が利用可能なCD81モノクローナル抗体(5A6)は、多様な実験ニーズに対応する多彩なオプションを提供します。CD81を標的とすることで、研究者は免疫細胞の活性化、ウイルスの侵入メカニズム、細胞機能を司る複雑なシグナル伝達経路などの重要なプロセスを調査することができます。CD81の翻訳後修飾は、がん転移や免疫障害などの病態におけるCD81の役割を理解する上で特に重要であり、抗CD81抗体(5A6)は基礎研究および応用研究の両方に不可欠なツールとなっています。

試験・研究用以外には使用しないでください。 臨床及び体外診断には使用できません。

Alexa Fluor® はMolecular Probes Inc., OR., USAの商標です。

LI-COR® and Odyssey® はLI-COR Biosciencesの登録商標です。

注文情報

製品名カタログ #単位価格数量お気に入り

CD81 抗体 (5A6)

sc-23962
200 µg/ml
$316.00

CD81 (5A6): m-IgG Fc BP-HRP Bundle

sc-526643
200 µg Ab; 10 µg BP
$354.00

CD81 (5A6): m-IgG1 BP-HRP Bundle

sc-532016
200 µg Ab; 20 µg BP
$354.00

CD81 抗体 (5A6) AC

sc-23962 AC
500 µg/ml, 25% agarose
$416.00

CD81 抗体 (5A6) HRP

sc-23962 HRP
200 µg/ml
$316.00

CD81 抗体 (5A6) FITC

sc-23962 FITC
200 µg/ml
$330.00

CD81 抗体 (5A6) PE

sc-23962 PE
200 µg/ml
$343.00

CD81 抗体 (5A6) Alexa Fluor® 488

sc-23962 AF488
200 µg/ml
$357.00

CD81 抗体 (5A6) Alexa Fluor® 546

sc-23962 AF546
200 µg/ml
$357.00

CD81 抗体 (5A6) Alexa Fluor® 594

sc-23962 AF594
200 µg/ml
$357.00

CD81 抗体 (5A6) Alexa Fluor® 647

sc-23962 AF647
200 µg/ml
$357.00

CD81 抗体 (5A6) Alexa Fluor® 680

sc-23962 AF680
200 µg/ml
$357.00

CD81 抗体 (5A6) Alexa Fluor® 790

sc-23962 AF790
200 µg/ml
$357.00

What is the isoelectric point for CD-81 antibody?

質問者: Sumita
Thank you for your question. We do not characterize the isoelectric point of our antibodies.
回答者: BlakeJ
投稿日: 2025-07-23

Hello, I have used the CD81 antibody for western blot at 1:1500 in 5% MILK on exosomes from human plasma. Do you know why I get two bands, one at 23 kDa and another one at 48 kDa? Many thanks Juliana

質問者: Juliana Redondo
Thank you for your question. The expected molecular weight for CD81 is ~ 22-26 kDa. We have not tested this antibody specifically in human plasma, and have not observed a higher molecular weight signal at 48 kDa in the cell lines tested. CD81 has been reported to dimerize (PMID: 20375010) and that may be a consideration. We would also recommend including a secondary antibody control, where you omit the primary antibody from the incubation, to confirm if the higher molecular weight band might be due to non-specific binding of the secondary antibody alone.
回答者: Technical Support
投稿日: 2017-10-16

Hi, I have used this antibody at 1:100 concentration on a western blot (10% SDS-PAGE gel) loading some exosomal protein. Could you explain please why I get not only the 26 kDa band, but also a band at about 50 kDa? Thank you.

質問者: VivSal
Thank you for your question. We have not tested this antibody on exosomal protein, or observed a 50 kDa band in Western blot. We would recommend including a secondary antibody control (omit primary antibody) to identify if the 50 kDa band may be a result of non-specific binding of the secondary antibody alone. It could also be your secondary antibody binding to heavy chain IgG in your samples. Below is a link to our recommended protocol for Western blotting. Please contact our Technical Service Department if for additional support with troubleshooting the antibody. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
回答者: Technical Support
投稿日: 2017-10-16

I have tried using this antibody on a western blot (after running an SDS-PAGE gel) with a homemade K562 lysate as control and did not get a signal at 1:100 dilution. I tried it 3 times previously on other cell lines at 1:200 w/no success. Any suggestions?

質問者: Tali
Thank you for your question. If you have no signal, we would recommend verifying that your anti-mouse secondary is working well. We would also recommend including a loading control antibody (e.g., Actin or GAPDH) to ensure that your transfer conditions are good, as well as your signal detection conditions (e.g., chemiluminescence or other). We have successfully used this antibody for WB at a dilution of 1:200, loading 50 µg of K-562 whole cell lysate. A link to our complete recommended protocol is provided below. If you continue to have negative results, please contact our Technical Service Department so we can provide further troubleshooting support. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase. If we are unable to successfully troubleshoot your application we will send you a replacement antibody free of charge, or issue a credit or refund if that is not possible https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting .
回答者: Technical Support
投稿日: 2017-04-26

What concentration would you recommend using SC-20048 at, to detect a protein in paraffin-embedded human tissue? We have used 1:1000 successfully for western blots. Please can you reply at your earliest convenience.

質問者: Deborah Goberdhan
Thank you for your question! We recommend a starting dilution of 1:50 for IHC on paraffin embedded tissue sections. This is a starting dilution, so it is possible that you can use it much more diluted! We also recommend performing an antigen retrieval step, ideally sodium citrate with heat. You can find our recommended protocol here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
回答者: Tech Service
投稿日: 2017-02-22

1, What concentration do you recommend using SC-23962 on paraffin embedded human tissue sections? We have this antibody, so would like to try. ; 2. Do you think that SC-23962 FITC would work better and at what conc. for same application, ie IHC(P)?

質問者: Deborah Goberdhan
Thank you for your question! We recommend a starting dilution of 1:50 for IHC on paraffin embedded tissue sections. We do recommend performing an antigen retrieval step. You can find our recommended protocol here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining You can use the FITC conjugated form, although we do not generally use FITC conjugated antibodies for IHC in house, so I do not have test results for this. If you wish to try it, I would stick with the 1:50 starting dilution as well. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
回答者: Tech Service
投稿日: 2017-02-22

Can I use this antibody for staining of mouse tissue, since it is a mouse antibody?

質問者: Anna99
Yes. CD81 Antibody (5A6): sc-23962 is available conjugated to either phycoerythrin (sc-23962 PE) or fluoroscein (sc-23962 FITC), 200 µg/ml, for IF, IHC(P) and FCM.
回答者: Tech Service 5
投稿日: 2017-01-05
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によって、 5つ星のうち 4と評価されています。 Great antibodyI request a sample of the antibody and it worked well!
投稿日: 2023-07-31
によって、 5つ星のうち 4と評価されています。 buon funzionamentoHo effettuato un solo esperimento per verificare la reattività nel cane e ha funzionato
投稿日: 2023-07-14
によって、 5つ星のうち 1と評価されています。 WBでバンドが検出できませんでした。エクソソームサンプルでCD81のバンドが全く検出できませんでした。同じサンプルで他のCD81抗体を使うとCD81を検出できたのでサンプルは問題ないと考えています。
投稿日: 2021-09-09
によって、 5つ星のうち 2と評価されています。 No band in Western blotI cannot see any band in Western blot after a nice transfer (all MW completely transfered; blotting using 1:200 of this antibody and 2nd antibody sc-516102 at 1:1000) of exosomes isolated by ultracentrifugation and further separated by density centrifugation. Perhaps I have low concentration of exosomes, but I expected to get at least a faint band.
投稿日: 2021-05-25
によって、 5つ星のうち 5と評価されています。 Works well in western blotThe antibody detected CD81 in Mesenchymal stem cells at the expected molecular size.
投稿日: 2020-09-18
によって、 5つ星のうち 5と評価されています。 Tested on HFFF by FACSThis antibody was tested on HFFF cells by FACS and shown a very strong signal, even at dilution 1:100
投稿日: 2018-11-19
によって、 5つ星のうち 5と評価されています。 Excellent antibody for ICC stainingThis antibody is gorgeous for ICC staining, please see the attached confocal image. Highly recommended. I tried 1:50 dilution for the first time, and you may further dilute it to 1:100 or even 1:200 to decrease the background a little bit, and it should work even better. I feel the overall quality of SCBT antibodies is getting much better,
投稿日: 2018-11-08
によって、 5つ星のうち 5と評価されています。 likeMembrane localization observed by Immunofluorescence staining of methanol-fixed embedded HeLa cells, also good for staining.
投稿日: 2017-05-07
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CD81抗体(5A6)は、 14件のレビューによって、 5つ星のうち 4.1と 評価されています。
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