CD3-γ Antibody (H-6): sc-55562

CD3-γ Antibody (H-6) is a mouse monoclonal IgG1 antibody that detects CD3-γ in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). CD3-γ is a crucial component of the T cell receptor (TCR) complex, playing a vital role in T cell activation and signaling. Located on the surface of T cells, CD3-γ forms part of a multisubunit complex that transmits signals upon recognition of foreign antigens, thereby initiating an immune response. The structure of CD3-γ, which includes a single immunoreceptor tyrosine-based activation motif (ITAM), is essential for its function, as this allows for the recruitment of protein kinases like ZAP-70 and Syk, which are pivotal in propagating the activation signal within the cell. CD3-γ presence is not only important for T cell development and function but also serves as a marker for identifying T cells in various immunological studies. Anti-CD3-γ antibody (H-6) is therefore an invaluable tool for researchers investigating T cell biology and the immune response.

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CD3-γ Antibody (H-6) References:

1. The CD3-gamma delta epsilon transducing module mediates CD38-induced protein-tyrosine kinase and mitogen-activated protein kinase activation in Jurkat T cells.  |  Zubiaur, M., et al. 1999. J Biol Chem. 274: 20633-42. PMID: 10400695
2. Evaluation of T-cell receptor CD3+ gamma delta in gestational diabetes mellitus.  |  Lapolla, A., et al. 2000. Acta Diabetol. 37: 207-11. PMID: 11450505
3. Contribution of CD3 gamma to TCR regulation and signaling in human mature T lymphocytes.  |  Torres, PS., et al. 2002. Int Immunol. 14: 1357-67. PMID: 12407027
4. A conformational epitope expressed upon association of CD3-epsilon with either CD3-delta or CD3-gamma is the main target for recognition by anti-CD3 monoclonal antibodies.  |  Salmerón, A., et al. 1991. J Immunol. 147: 3047-52. PMID: 1717585
5. The CD3-gamma and CD3-delta subunits of the T cell antigen receptor can be expressed within distinct functional TCR/CD3 complexes.  |  Alarcón, B., et al. 1991. EMBO J. 10: 903-12. PMID: 1826255
6. Characterization of thymus-derived lymphocytes expressing Ti alpha-beta CD3 gamma delta epsilon zeta-zeta, Ti alpha-beta CD3 gamma delta epsilon eta-eta or Ti alpha-beta CD3 gamma delta epsilon zeta-zeta/zeta-eta antigen receptor isoforms: analysis by gene transfection.  |  Clayton, LK., et al. 1990. J Exp Med. 172: 1243-53. PMID: 2145389
7. Human T-cell receptor CD3-gamma (CD3G)/MspI DNA polymorphism.  |  Charmley, P., et al. 1989. Nucleic Acids Res. 17: 2372. PMID: 2468133
8. Assembly of the human T cell receptor-CD3 complex takes place in the endoplasmic reticulum and involves intermediary complexes between the CD3-gamma.delta.epsilon core and single T cell receptor alpha or beta chains.  |  Alarcon, B., et al. 1988. J Biol Chem. 263: 2953-61. PMID: 2963821
9. CD3 gamma, CD3 delta, and CD3 zeta mRNA in adult human marrow hematopoietic progenitors correlates with surface CD2 and CD7 expression.  |  Long, H., et al. 1996. Exp Hematol. 24: 1402-8. PMID: 8913286
10. Construction of retroviral vectors carrying human CD3 gamma cDNA and reconstitution of CD3 gamma expression and T cell receptor surface expression and function in a CD3 gamma-deficient mutant T cell line.  |  Sun, JY., et al. 1997. Hum Gene Ther. 8: 1041-8. PMID: 9189762

The preferred CD3-δ antibody is CD3-δ Antibody (F-1): sc-137137.