caspase-9 Antibody (96.1.23): sc-56076

Caspase-9 Antibody (96.1.23) is a mouse monoclonal IgG1 kappa light chain antibody that detects caspase-9 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-caspase-9 antibody (96.1.23) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Caspase-9 plays a crucial role in the apoptotic pathway, acting as an initiator caspase that activates downstream effector caspases, such as caspase-3 and caspase-7, thereby facilitating programmed cell death. This process maintains cellular homeostasis and eliminates damaged or unwanted cells, which is particularly important in cancer and neurodegenerative diseases. Caspase-9 activation is tightly regulated and occurs in response to apoptotic signals, highlighting its significance in cellular response to stress and damage. Understanding caspase-9 function and interactions with other proteins in the apoptotic cascade can provide insights into therapeutic strategies aimed at modulating apoptosis in various diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

caspase-9 Antibody (96.1.23) References:

1. Regulation of the Apaf-1/caspase-9 apoptosome by caspase-3 and XIAP.  |  Zou, H., et al. 2003. J Biol Chem. 278: 8091-8. PMID: 12506111
2. HAX-1 represses postmitochondrial caspase-9 activation and cell death during hypoxia-reoxygenation.  |  Shaw, J. and Kirshenbaum, LA. 2006. Circ Res. 99: 336-8. PMID: 16917098
3. A Rapamycin-Activated Caspase 9-Based Suicide Gene.  |  Stavrou, M., et al. 2018. Mol Ther. 26: 1266-1276. PMID: 29661681
4. Caspase-9 Is a Positive Regulator of Osteoblastic Cell Migration Identified by diaPASEF Proteomics.  |  Říhová, K., et al. 2024. J Proteome Res. 23: 2999-3011. PMID: 38498986
5. Post-translational modification of poly(ADP-ribose) polymerase induced by DNA strand breaks.  |  Lindahl, T., et al. 1995. Trends Biochem Sci. 20: 405-11. PMID: 8533153
6. ICE-LAP3, a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis.  |  Duan, H., et al. 1996. J Biol Chem. 271: 1621-5. PMID: 8576161
7. Apopain/CPP32 cleaves proteins that are essential for cellular repair: a fundamental principle of apoptotic death.  |  Casciola-Rosen, L., et al. 1996. J Exp Med. 183: 1957-64. PMID: 8642305
8. ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B.  |  Duan, H., et al. 1996. J Biol Chem. 271: 16720-4. PMID: 8663294
9. Cleavage of lamin A by Mch2 alpha but not CPP32: multiple interleukin 1 beta-converting enzyme-related proteases with distinct substrate recognition properties are active in apoptosis.  |  Takahashi, A., et al. 1996. Proc Natl Acad Sci U S A. 93: 8395-400. PMID: 8710882
10. In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains.  |  Fernandes-Alnemri, T., et al. 1996. Proc Natl Acad Sci U S A. 93: 7464-9. PMID: 8755496
11. The expression of poly(ADP-ribose) polymerase during differentiation-linked DNA replication reveals that it is a component of the multiprotein DNA replication complex.  |  Simbulan-Rosenthal, CM., et al. 1996. Biochemistry. 35: 11622-33. PMID: 8794742
12. Caspases: the executioners of apoptosis.  |  Cohen, GM. 1997. Biochem J. 326 (Pt 1): 1-16. PMID: 9337844

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-caspase-9 Antibody (96.1.23) has 114 citations in a variety of scientific publications.

Primary antibodies like Anti-caspase-9 Antibody (96.1.23) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

caspase-9 Antibody (96.1.23) Specifications

Alternate Product Name: caspase-9

Product Clone Name: 96.1.23

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: caspase-9

Epitope Species: human

Product Reactivity/Detection: caspase-9

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 114 citations in various scientific publications

Available Conjugates/Forms for caspase-9 Antibody (96.1.23): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin