BUB1 Antibody (B-3): sc-365685

BUB1 Antibody (B-3) is a mouse monoclonal IgG3 kappa light chain antibody that detects BUB1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-BUB1 antibody (B-3) is available as the non-conjugated form. BUB1 protein plays a critical role in the mitotic checkpoint, a crucial mechanism that ensures proper chromosome segregation during cell division. BUB1 protein is located at the kinetochores, the protein structures on chromosomes where spindle fibers attach during mitosis. Such localization allows BUB1 protein to monitor microtubule attachment to kinetochores, ensuring chromosomes are correctly aligned before cell proceeds to anaphase. When BUB1 protein detects misalignment or improper attachment, a checkpoint response activates to halt the cell cycle, preventing potential genomic instability and aneuploidy. BUB1 protein′s role in maintaining genomic integrity highlights potential applications in cancer research, where mitotic checkpoint dysregulation can lead to tumorigenesis.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

BUB1 Antibody (B-3) References:

1. Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC.  |  Chan, GK., et al. 1999. J Cell Biol. 146: 941-54. PMID: 10477750
2. BUBR1 phosphorylation is regulated during mitotic checkpoint activation.  |  Li, W., et al. 1999. Cell Growth Differ. 10: 769-75. PMID: 10593653
3. Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis.  |  Kalitsis, P., et al. 2000. Genes Dev. 14: 2277-82. PMID: 10995385
4. CENP-E as an essential component of the mitotic checkpoint in vitro.  |  Abrieu, A., et al. 2000. Cell. 102: 817-26. PMID: 11030625
5. Identification of a novel gene--SSK1--in human endothelial cells exposed to shear stress.  |  Donadelli, R., et al. 1998. Biochem Biophys Res Commun. 246: 881-7. PMID: 9618306
6. The hBUB1 and hBUBR1 kinases sequentially assemble onto kinetochores during prophase with hBUBR1 concentrating at the kinetochore plates in mitosis.  |  Jablonski, SA., et al. 1998. Chromosoma. 107: 386-96. PMID: 9914370

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BUB1 Antibody (B-3) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-BUB1 Antibody (B-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

BUB1 Antibody (B-3) Specifications

Alternate Product Name: BUB1

Product Clone Name: B-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 786-1085

Epitope Target: BUB1

Epitope Species: human

Product Reactivity/Detection: BUB1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for BUB1 Antibody (B-3): N/A