BAM32 Antibody (E-10): sc-133166
- BAM32 Antibody (E-10) is a mouse monoclonal IgG1 κ BAM32 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-280 representing full length BAM32 of human origin
- BAM32 Antibody (E-10) is recommended for detection of BAM32 of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-BAM32 Antibody (E-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for BAM32 Antibody (E-10) for WB and IHC(P) applications. These reagents are now offered in bundles with BAM32 Antibody (E-10) (see ordering information below).
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SEE ALSO...
BAM32 Antibody (E-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects BAM32 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). BAM32 (E-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The B cell adapter molecule (BAM32), also known as dual adapter for phosphotyrosine and 3-phosphoinositide (DAPP1) or B lymphocyte adapter protein, plays a vital role in regulating B cell antigen receptor signaling. BAM32 is primarily located in the cytoplasm but can translocate to the cell membrane upon B cell activation, which is crucial for effective immune responses. BAM32 contains one pleckstrin homology (PH) domain and one Src homology 2 (SH2) domain, allowing BAM32 to interact with phosphoinositides and phospholipase C gamma 2, facilitating downstream signaling pathways. BAM32 is predominantly expressed in the placenta and lung tissues, with detectable levels in the heart, liver, pancreas, and brain, highlighting BAM32′s importance in various physiological processes. The phosphorylation of BAM32 on tyrosine residues upon B cell activation underscores BAM32′s role in modulating immune signaling, making BAM32 a significant target for research in immunology and related fields.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
BAM32 Antibody (E-10) References:
- Structural basis for discrimination of 3-phosphoinositides by pleckstrin homology domains. | Ferguson, KM., et al. 2000. Mol Cell. 6: 373-84. PMID: 10983984
- The B cell SH2/PH domain-containing adaptor Bam32/DAPP1 is required for T cell-independent II antigen responses. | Fournier, E., et al. 2003. Curr Biol. 13: 1858-66. PMID: 14588241
- Branches of the B cell antigen receptor pathway are directed by protein conduits Bam32 and Carma1. | Niiro, H. and Clark, EA. 2003. Immunity. 19: 637-40. PMID: 14614850
- The adaptor protein Bam32 regulates Rac1 activation and actin remodeling through a phosphorylation-dependent mechanism. | Allam, A., et al. 2004. J Biol Chem. 279: 39775-82. PMID: 15247305
- The B lymphocyte adaptor molecule of 32 kilodaltons (Bam32) regulates B cell antigen receptor internalization. | Niiro, H., et al. 2004. J Immunol. 173: 5601-9. PMID: 15494510
- Role of the adaptor proteins Bam32, TAPP1 and TAPP2 in lymphocyte activation. | Allam, A. and Marshall, AJ. 2005. Immunol Lett. 97: 7-17. PMID: 15626471
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BAM32 Antibody (E-10) | sc-133166 | 200 µg/ml | $322.00 | |||
BAM32 Antibody (E-10): m-IgG Fc BP-HRP Bundle | sc-528756 | 200 µg Ab; 10 µg BP | $361.00 | |||
BAM32 Antibody (E-10): m-IgGκ BP-HRP Bundle | sc-521257 | 200 µg Ab, 40 µg BP | $361.00 | |||
BAM32 Antibody (E-10): m-IgG1 BP-HRP Bundle | sc-543113 | 200 µg Ab; 20 µg BP | $361.00 | |||
BAM32 Antibody (E-10) AC | sc-133166 AC | 500 µg/ml, 25% agarose | $424.00 | |||
BAM32 Antibody (E-10) HRP | sc-133166 HRP | 200 µg/ml | $322.00 | |||
BAM32 Antibody (E-10) FITC | sc-133166 FITC | 200 µg/ml | $336.00 | |||
BAM32 Antibody (E-10) PE | sc-133166 PE | 200 µg/ml | $349.00 | |||
BAM32 Antibody (E-10) Alexa Fluor® 488 | sc-133166 AF488 | 200 µg/ml | $364.00 | |||
BAM32 Antibody (E-10) Alexa Fluor® 546 | sc-133166 AF546 | 200 µg/ml | $364.00 | |||
BAM32 Antibody (E-10) Alexa Fluor® 594 | sc-133166 AF594 | 200 µg/ml | $364.00 | |||
BAM32 Antibody (E-10) Alexa Fluor® 647 | sc-133166 AF647 | 200 µg/ml | $364.00 | |||
BAM32 Antibody (E-10) Alexa Fluor® 680 | sc-133166 AF680 | 200 µg/ml | $364.00 | |||
BAM32 Antibody (E-10) Alexa Fluor® 790 | sc-133166 AF790 | 200 µg/ml | $364.00 |