ADAR1 Antibody (15.8.6): sc-73408

ADAR1 Antibody (15.8.6) is a mouse monoclonal IgG1 kappa light chain antibody that detects ADAR1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-ADAR1 antibody (15.8.6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The RNA-specific adenosine deaminase, ADAR1, plays a crucial role in RNA editing by converting adenosine to inosine in double-stranded RNA, which is essential for the regulation of gene expression and maintenance of RNA stability. This post-translational modification influences the coding potential of mRNA and functionality of non-coding RNAs, thereby impacting various cellular processes and responses to environmental stimuli. ADAR1 is expressed from an interferon-response promoter and features a Z-DNA/Z-RNA binding domain at its N-terminus, which is important for structured RNA interaction. ADAR1 co-localizes with SUMO-1 in a distinct subnucleolar region, and nuclear localization is regulated by a nucleolar localization signal and nuclear exporter signal. ADAR1′s ability to bind short interfering RNA (siRNA) suggests a role in modulating gene silencing effectiveness in mammalian cells, highlighting significance in RNA biology and therapeutic applications.

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ADAR1 Antibody (15.8.6) References:

1. Requirement of the RNA editing deaminase ADAR1 gene for embryonic erythropoiesis.  |  Wang, Q., et al. 2000. Science. 290: 1765-8. PMID: 11099415
2. RNA editing activity is associated with splicing factors in lnRNP particles: The nuclear pre-mRNA processing machinery.  |  Raitskin, O., et al. 2001. Proc Natl Acad Sci U S A. 98: 6571-6. PMID: 11381114
3. Nucleocytoplasmic distribution of human RNA-editing enzyme ADAR1 is modulated by double-stranded RNA-binding domains, a leucine-rich export signal, and a putative dimerization domain.  |  Strehblow, A., et al. 2002. Mol Biol Cell. 13: 3822-35. PMID: 12429827
4. Induction of protein translation by ADAR1 within living cell nuclei is not dependent on RNA editing.  |  Herbert, A., et al. 2002. Mol Cell. 10: 1235-46. PMID: 12453429
5. Requirement of dimerization for RNA editing activity of adenosine deaminases acting on RNA.  |  Cho, DS., et al. 2003. J Biol Chem. 278: 17093-102. PMID: 12618436
6. Subcellular distribution of ADAR1 isoforms is synergistically determined by three nuclear discrimination signals and a regulatory motif.  |  Nie, Y., et al. 2004. J Biol Chem. 279: 13249-55. PMID: 14711814
7. ADAR1 RNA deaminase limits short interfering RNA efficacy in mammalian cells.  |  Yang, W., et al. 2005. J Biol Chem. 280: 3946-53. PMID: 15556947
8. Expression of interferon-inducible RNA adenosine deaminase ADAR1 during pathogen infection and mouse embryo development involves tissue-selective promoter utilization and alternative splicing.  |  George, CX., et al. 2005. J Biol Chem. 280: 15020-8. PMID: 15677478
9. Chromosomal storage of the RNA-editing enzyme ADAR1 in Xenopus oocytes.  |  Sallacz, NB. and Jantsch, MF. 2005. Mol Biol Cell. 16: 3377-86. PMID: 15843431
10. The crystal structure of the Zbeta domain of the RNA-editing enzyme ADAR1 reveals distinct conserved surfaces among Z-domains.  |  Athanasiadis, A., et al. 2005. J Mol Biol. 351: 496-507. PMID: 16023667
11. ADAR1 interacts with NF90 through double-stranded RNA and regulates NF90-mediated gene expression independently of RNA editing.  |  Nie, Y., et al. 2005. Mol Cell Biol. 25: 6956-63. PMID: 16055709
12. SUMO-1 modification alters ADAR1 editing activity.  |  Desterro, JM., et al. 2005. Mol Biol Cell. 16: 5115-26. PMID: 16120648
13. Modulation of ADAR1 editing activity by Z-RNA in vitro.  |  Koeris, M., et al. 2005. Nucleic Acids Res. 33: 5362-70. PMID: 16177183

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ADAR1 Antibody (15.8.6) has 89 citations in a variety of scientific publications.

Primary antibodies like Anti-ADAR1 Antibody (15.8.6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ADAR1 Antibody (15.8.6) Specifications

Alternate Product Name: ADAR1

Product Clone Name: 15.8.6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 440-826

Epitope Target: ADAR1

Epitope Species: human

Product Reactivity/Detection: native and recombinant ADAR1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of GST-tagged recombinant protein corresponding to human ADAR1.

Research Citations: 89 citations in various scientific publications

Available Conjugates/Forms for ADAR1 Antibody (15.8.6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred ADAR1 antibody is ADAR1 Antibody (D-8): sc-271854.