SOCS-3 Antibody (G-5): sc-518020
- SOCS-3 Antibody (G-5) is a mouse monoclonal IgG1 κ, cited in 8 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 200-225 at the C-terminus of SOCS-3 of mouse origin
- Anti-SOCS-3 Antibody (G-5) is recommended for detection of SOCS-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-SOCS-3 Antibody (G-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SOCS-3 Antibody (G-5) for WB and IHC(P) applications. These reagents are now offered in bundles with SOCS-3 Antibody (G-5) (see ordering information below).
SOCS-3 Antibody (G-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects SOCS-3 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). SOCS-3 (G-5) antibody is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. SOCS-3 plays a crucial role in the negative regulation of cytokine signaling, which is vital for maintaining immune homeostasis and preventing excessive inflammatory responses. SOCS-3 is primarily located in the cytoplasm, where SOCS-3 interacts with various signaling pathways, including those activated by interleukin-6 (IL-6). SOCS-3′s ability to inhibit these pathways is essential for controlling the duration and intensity of cytokine signaling, thereby preventing chronic inflammation and autoimmune diseases. Additionally, SOCS-3 undergoes post-translational modifications, such as phosphorylation, which can influence SOCS-3′s stability and activity, further underscoring SOCS-3′s importance in cellular signaling networks.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SOCS-3 Antibody (G-5) References:
- Suppressor of cytokine signaling (SOCS)-3 protein interacts with the insulin-like growth factor-I receptor. | Dey, BR., et al. 2000. Biochem Biophys Res Commun. 278: 38-43. PMID: 11071852
- SOCS-3 inhibits insulin signaling and is up-regulated in response to tumor necrosis factor-alpha in the adipose tissue of obese mice. | Emanuelli, B., et al. 2001. J Biol Chem. 276: 47944-9. PMID: 11604392
- SOCS-1 and SOCS-3 block insulin signaling by ubiquitin-mediated degradation of IRS1 and IRS2. | Rui, L., et al. 2002. J Biol Chem. 277: 42394-8. PMID: 12228220
- SOCS-3 is involved in the downregulation of the acute insulin-like effects of growth hormone in rat adipocytes by inhibition of Jak2/IRS-1 signaling. | Ridderstråle, M., et al. 2003. Horm Metab Res. 35: 169-77. PMID: 12734778
- Sepsis-induced SOCS-3 expression is immunologically restricted to phagocytes. | Grutkoski, PS., et al. 2003. J Leukoc Biol. 74: 916-22. PMID: 12960286
- SOCS-3 inhibits IL-12-induced STAT4 activation by binding through its SH2 domain to the STAT4 docking site in the IL-12 receptor beta2 subunit. | Yamamoto, K., et al. 2003. Biochem Biophys Res Commun. 310: 1188-93. PMID: 14559241
- Suppressor of cytokine signaling 1 (SOCS-1) and SOCS-3 cause insulin resistance through inhibition of tyrosine phosphorylation of insulin receptor substrate proteins by discrete mechanisms. | Ueki, K., et al. 2004. Mol Cell Biol. 24: 5434-46. PMID: 15169905
- SOCS-3 is frequently methylated in head and neck squamous cell carcinoma and its precursor lesions and causes growth inhibition. | Weber, A., et al. 2005. Oncogene. 24: 6699-708. PMID: 16007169
- Effects of ischemic preconditioning on myocardium Caspase-3, SOCS-1, SOCS-3, TNF-α and IL-6 mRNA expression levels in myocardium IR rats. | Ma, J., et al. 2013. Mol Biol Rep. 40: 5741-8. PMID: 24091940
- Kidney-Replenishing Herb Induces SOCS-3 Expression via ERK/MAPK Pathway and Improves Growth of the First-Trimester Human Trophoblast Cells. | Xing, CY., et al. 2017. Evid Based Complement Alternat Med. 2017: 2473431. PMID: 29234375
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SOCS-3 Antibody (G-5) | sc-518020 | 200 µg/ml | $322.00 | |||
SOCS-3 Antibody (G-5): m-IgG Fc BP-HRP Bundle | sc-531596 | 200 µg Ab; 10 µg BP | $361.00 | |||
SOCS-3 Antibody (G-5): m-IgGκ BP-HRP Bundle | sc-525328 | 200 µg Ab, 40 µg BP | $361.00 | |||
SOCS-3 Antibody (G-5): m-IgG1 BP-HRP Bundle | sc-544648 | 200 µg Ab; 20 µg BP | $361.00 | |||
SOCS-3 Antibody (G-5) AC | sc-518020 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SOCS-3 Antibody (G-5) HRP | sc-518020 HRP | 200 µg/ml | $322.00 | |||
SOCS-3 Antibody (G-5) FITC | sc-518020 FITC | 200 µg/ml | $336.00 | |||
SOCS-3 Antibody (G-5) PE | sc-518020 PE | 200 µg/ml | $349.00 | |||
SOCS-3 Antibody (G-5) Alexa Fluor® 488 | sc-518020 AF488 | 200 µg/ml | $364.00 | |||
SOCS-3 Antibody (G-5) Alexa Fluor® 546 | sc-518020 AF546 | 200 µg/ml | $364.00 | |||
SOCS-3 Antibody (G-5) Alexa Fluor® 594 | sc-518020 AF594 | 200 µg/ml | $364.00 | |||
SOCS-3 Antibody (G-5) Alexa Fluor® 647 | sc-518020 AF647 | 200 µg/ml | $364.00 | |||
SOCS-3 Antibody (G-5) Alexa Fluor® 680 | sc-518020 AF680 | 200 µg/ml | $364.00 | |||
SOCS-3 Antibody (G-5) Alexa Fluor® 790 | sc-518020 AF790 | 200 µg/ml | $364.00 |