Anticorpo PTEN (A2B1) raccomandato per il rilevamento di PTEN di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA

Anticorpo PTEN (A2B1): sc-7974

Anticorpo PTEN (A2B1) valutazione 4.7 di 5 di 69.
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Schede tecniche

    • Anti-PTEN Antibody A2B1 è un monoclonale di topo IgG1 κ PTEN antibody, citato in 456 pubblicazioni, fornito in 200 µg/ml
    • generato contro aminoacidi 388-400 mapping al C-terminus dell´ PTEN di origine human
    • PTEN Antibody (A2B1) é raccomandato per il rilevamento di PTEN di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA
    • PTEN Antibody (A2B1) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Contattaci per ricevere un FREE 10 µg sample di PTEN (A2B1): sc-7974.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for PTEN Antibody (A2B1) for WB and IHC(P) applications. These reagents are now offered in bundles with PTEN Antibody (A2B1) (see ordering information below).

Good morning. I used PTEN, sc7974 on prostate tissue. It gave me positive staining in extraglandular tissue and not in glandular structures. Could you guide me for proper steps to obtain immunostaining in the cells of prostatic glands? Thank you

Richiesta da: Nhelal
Thank you for your question. Please contact our Technical Service Department or your local distributor for assistance in troubleshooting your IHC reuslts with this antibody.
Risposta da: Tech Service
Data di pubblicazione: 2019-09-26

Good Morning. I want to use PTEN monoclonal for IHC(P) on prostate tissue. I need your advice, Which product is more suitable: sc7974, sc133242, or sc393186? thanks

Richiesta da: Nhelal
Thank you for your question. All three of these PTEN antibodies are recommended for IHC(P) applications. PTEN Antibody (A2B1): sc-7974 is featured in over 300 publications, which you can find right here on the product page.
Risposta da: Tech Support Europe
Data di pubblicazione: 2019-09-26

Hallo, when I tried to WB with BT-549(PTEN null as negative control) and Hela(Positive control), I find that non specific bands near 60 kDa in both BT-549 and Hela.  Also, in this datasheet, there are 3 bands in Hela. Which is tha authentic bands?

Richiesta da: taro333
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Risposta da: Tech Service
Data di pubblicazione: 2019-02-18

Hello, we are looking for PTEN Antibody to stained our sample which is goat ovary tissue in form of IHC-P. I wish you give me information that PTEN A2B1 (or any of your product) will be reactive to our sample. Thankyou.

Richiesta da: Dimas Reza
Thank you for your question. Unfortunately, we are not aware of any citations using this, or any of our other PTEN antibodies for detection in goat samples. Please contact our Technical Service Department with the protein sequence or accession number for the goat PTEN protein and we would be happy to help you perform sequence analysis to predict any antibodies that may cross-react with your species of interest.
Risposta da: Technical Support
Data di pubblicazione: 2018-12-11

WB的稀释比例是多少

Richiesta da: 2333333
感谢您的提问。推荐用于WB的稀释度是1:100-1:1000,关于此问题的详细细节,欢迎通过:电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”,联系我们的技术支持部门。 Thank you for your inquiry. The recommended dilution for WB is 1:100-1:1000. For more detailed information about this question, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website.
Risposta da: Technical Support 15
Data di pubblicazione: 2018-02-01

Good afternoon, Would it be possible to get the mentioned free 10 µg Sample of the anti-PTEN antibody PTEN (A2B1): sc-7974 ? Best regards, Ana Lopes

Richiesta da: Ana Lopes
Thank you for your question. Yes, we are happy to provide you with a free 10 µg sample of this antibody. Please call our European Support office toll-free at 00800 4573 8000, email [email protected], or contact us by live chat to request the free sample.
Risposta da: Technical Support
Data di pubblicazione: 2017-07-28

I'll soon use Pten antibody for IF of Pten expression in mouse tissues so that I wonder if there's big difference between sc-7974 and sc-133197. Please advise.

Richiesta da: Jessica77
Thank you for your question. The main difference between PTEN (A2B1): sc-7974 and PTEN (B-1): sc-133197 is the immunogen sequence that they were raised against. Both antibodies are recommended for use in mouse tissue by IF. If you require further assistance, please contact Technical Service.
Risposta da: Santa Cruz TS
Data di pubblicazione: 2017-05-19

Hi, I am interested in purchasing the PTEN: sc-7974 AC for an IP protocol I am putting together. I was wondering if you could recommend any good controls?

Richiesta da: arwhite
Thank you for your question. This monoclonal PTEN Antibody (A2B1): sc-7974, is available conjugated to agarose (sc-7974 AC) for immunoprecipitation. We would recommend some of the below products as negative and positive controls for this research application: normal mouse IgG-AC: sc-2343 to be used as an negative control MCF7 (IP) Cell Lysate: sc-24793 to be used as a positive control A-431 (IP) Cell Lysate: sc-24781 to be used as a positive control Below is a link to our recommended protocol which also highlights some additional controls and support products available: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Risposta da: Technical Support
Data di pubblicazione: 2017-01-27
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Rated 4 di 5 di da Good detection I detected the bands but they were not very intense. Maybe it was due to the cell type (lung adenocarcinoma)
Data di pubblicazione: 2021-03-17
Rated 5 di 5 di da Good this antibody works well for western blot with MEF
Data di pubblicazione: 2021-03-10
Rated 5 di 5 di da good ab Got this ab as a free sample, works very fine on mouse brain lysates. Clear cut band at the right molecular weight, extremely satisfied.
Data di pubblicazione: 2021-02-03
Rated 5 di 5 di da Great antibody You can get great western blot images by using this antibody.
Data di pubblicazione: 2020-08-13
Rated 5 di 5 di da good antibody for WB good antibody for WB. PTEN shows up clear at where it should be.
Data di pubblicazione: 2019-09-23
Rated 5 di 5 di da WB和IP实验结果都很好 我们用了SC-7974和SC-7974(AC),这是我们实验室最常用的抗体之一,SC-7974(AC)功能很强大,可以用于IP实验并避免重链干扰(用Glycin-HCl洗脱),是研究PTEN蛋白修饰和相互作用蛋白的利器。
Data di pubblicazione: 2019-07-02
Rated 5 di 5 di da Great antibody for WBs I have used this antibody in 1:500 dilution for western blotting using cancer cells and was very impressed with the specificity and quality of signal
Data di pubblicazione: 2019-06-15
Rated 5 di 5 di da It works great for western blot. sc-7974 works great for western blot with HUVECs cell lines.
Data di pubblicazione: 2018-11-12
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