Nek2 Antibody (D-8): sc-55601
- Nek2 Antibody (D-8) is a mouse monoclonal IgG2b κ Nek2 antibody, cited in 33 publications, provided at 200 µg/ml
- raised against amino acids 211-445 mapping at the C-terminus of Nek2 of human origin
- Nek2 Antibody (D-8) is recommended for detection of Nek2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Nek2 Antibody (D-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Nek2 Antibody (D-8) for WB and IHC(P) applications. These reagents are now offered in bundles with Nek2 Antibody (D-8) (see ordering information below).
Nek2 Antibody (D-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects Nek2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Nek2 antibody (D-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nek2 plays a crucial role in cell cycle regulation, particularly during the transition from G2 phase to mitosis, where expression peaks. This regulation is vital for proper cell division, as aberrations in Nek2 activity can lead to genomic instability and contribute to tumorigenesis. Additionally, Nek2 is closely related to NIMA protein found in Aspergillus nidulans, which is essential for mitotic entry, highlighting evolutionary conservation of function. In mammalian systems, Nek2 expression is tightly regulated, and dysregulation has been implicated in various cancers, making Nek2 a significant target for research and therapeutic interventions. Nek2 (D-8) antibody effectively detects across multiple species and applications, making this tool invaluable for researchers studying cell cycle dynamics and related pathologies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Nek2 Antibody (D-8) References:
- Cloning and characterization of the murine Nek3 protein kinase, a novel member of the NIMA family of putative cell cycle regulators. | Tanaka, K. and Nigg, EA. 1999. J Biol Chem. 274: 13491-7. PMID: 10224116
- NIMA-related kinases: isolation and characterization of murine nek3 and nek4 cDNAs, and chromosomal localization of nek1, nek2 and nek3. | Chen, A., et al. 1999. Gene. 234: 127-37. PMID: 10393247
- A mammalian dual specificity protein kinase, Nek1, is related to the NIMA cell cycle regulator and highly expressed in meiotic germ cells. | Letwin, K., et al. 1992. EMBO J. 11: 3521-31. PMID: 1382974
- Mitotic induction and maintenance by overexpression of a G2-specific gene that encodes a potential protein kinase. | Osmani, SA., et al. 1988. Cell. 53: 237-44. PMID: 3359487
- Cell cycle-dependent expression of Nek2, a novel human protein kinase related to the NIMA mitotic regulator of Aspergillus nidulans. | Schultz, SJ., et al. 1994. Cell Growth Differ. 5: 625-35. PMID: 7522034
- The NIMA-related kinase 2, Nek2, is expressed in specific stages of the meiotic cell cycle and associates with meiotic chromosomes. | Rhee, K. and Wolgemuth, DJ. 1997. Development. 124: 2167-77. PMID: 9187143
- Characterization of mammalian NIMA-related kinases. | Fry, AM. and Nigg, EA. 1997. Methods Enzymol. 283: 270-82. PMID: 9251026
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nek2 Antibody (D-8) | sc-55601 | 200 µg/ml | $322.00 | |||
Nek2 Antibody (D-8): m-IgG Fc BP-HRP Bundle | sc-528610 | 200 µg Ab; 10 µg BP | $361.00 | |||
Nek2 Antibody (D-8): m-IgGκ BP-HRP Bundle | sc-521030 | 200 µg Ab, 40 µg BP | $361.00 | |||
Nek2 Antibody (D-8): m-IgG2b BP-HRP Bundle | sc-550057 | 200 µg Ab; 10 µg BP | $361.00 | |||
Nek2 Antibody (D-8) AC | sc-55601 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Nek2 Antibody (D-8) HRP | sc-55601 HRP | 200 µg/ml | $322.00 | |||
Nek2 Antibody (D-8) FITC | sc-55601 FITC | 200 µg/ml | $336.00 | |||
Nek2 Antibody (D-8) PE | sc-55601 PE | 200 µg/ml | $349.00 | |||
Nek2 Antibody (D-8) Alexa Fluor® 488 | sc-55601 AF488 | 200 µg/ml | $364.00 | |||
Nek2 Antibody (D-8) Alexa Fluor® 546 | sc-55601 AF546 | 200 µg/ml | $364.00 | |||
Nek2 Antibody (D-8) Alexa Fluor® 594 | sc-55601 AF594 | 200 µg/ml | $364.00 | |||
Nek2 Antibody (D-8) Alexa Fluor® 647 | sc-55601 AF647 | 200 µg/ml | $364.00 | |||
Nek2 Antibody (D-8) Alexa Fluor® 680 | sc-55601 AF680 | 200 µg/ml | $364.00 | |||
Nek2 Antibody (D-8) Alexa Fluor® 790 | sc-55601 AF790 | 200 µg/ml | $364.00 |