NAGA Antibody (F-1): sc-393485

NAGA Antibody (F-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects NAGA protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-NAGA antibody (F-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NAGA protein, also known as alpha-galactosidase B or GALB, is a 411 amino acid lysosomal enzyme that plays a vital role in the breakdown of glycolipids, which are essential for cellular membrane integrity and signaling. NAGA is located primarily in lysosomes, where NAGA functions to cleave alpha-N-acetylgalactosaminyl groups from glycoconjugates, facilitating the degradation of complex carbohydrates. Proper functioning of NAGA is crucial, as defects in this enzyme can lead to the accumulation of glycosphingolipids, resulting in severe neurological disorders such as Schindler disease, which presents with varying degrees of intellectual impairment and other systemic manifestations. Mapping to human chromosome 22q13, mutations in the NAGA gene are responsible for this autosomal recessive disorder, highlighting NAGA′s importance in maintaining cellular homeostasis and the potential consequences of dysfunction.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

NAGA Antibody (F-1) References:

1. A new case of alpha-N-acetylgalactosaminidase deficiency with angiokeratoma corporis diffusum, with Ménière's syndrome and without mental retardation.  |  Kodama, K., et al. 2001. Br J Dermatol. 144: 363-8. PMID: 11251574
2. Human alpha-N-acetylgalactosaminidase (alpha-NAGA) deficiency: no association with neuroaxonal dystrophy?  |  Bakker, HD., et al. 2001. Eur J Hum Genet. 9: 91-6. PMID: 11313741
3. Localization of a gene for human alpha-galactosidase B (= n-acetyl-alpha-d-galactosaminidase) on chromosome 22.  |  de Groot, PG., et al. 1978. Hum Genet. 44: 305-12. PMID: 215508
4. Schindler disease: the molecular lesion in the alpha-N-acetylgalactosaminidase gene that causes an infantile neuroaxonal dystrophy.  |  Wang, AM., et al. 1990. J Clin Invest. 86: 1752-6. PMID: 2243144
5. Regional localization of the genes coding for human ACO2, ARSA, and NAGA on chromosome 22.  |  Geurts van Kessel, AH., et al. 1980. Cytogenet Cell Genet. 28: 169-72. PMID: 7192199
6. alpha-N-acetylgalactosaminidase deficiency with mild clinical manifestations and difficult biochemical diagnosis.  |  de Jong, J., et al. 1994. J Pediatr. 125: 385-91. PMID: 8071745
7. Human alpha-N-acetylgalactosaminidase (alpha-NAGA) deficiency: new mutations and the paradox between genotype and phenotype.  |  Keulemans, JL., et al. 1996. J Med Genet. 33: 458-64. PMID: 8782044

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NAGA Antibody (F-1) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-NAGA Antibody (F-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NAGA Antibody (F-1) Specifications

Alternate Product Name: NAGA

Product Clone Name: F-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 335-411

Epitope Target: NAGA

Epitope Species: human

Product Reactivity/Detection: NAGA

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for NAGA Antibody (F-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin