MHC class II Antibody (Y-Ae): sc-32247
- MHC class II Antibody (Y-Ae) is a mouse monoclonal IgG2b λ MHC class II antibody, cited in 16 publications, provided at 200 µg/ml
- raised against lipopolysaccharide activated spleen cells of mouse origin
- MHC class II Antibody (Y-Ae) is recommended for detection of splenic B cells expressing the I-Ab MHC class II molecules complexed to the 52-68 fragment of the α chain of I-E class II molecules (the Eα 52-68 peptide) of mouse origin by WB, IP, IF, IHC(P) and FCM
- Anti-MHC class II Antibody (Y-Ae) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for MHC class II Antibody (Y-Ae). This work is in progress.
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SEE ALSO...
MHC class II Antibody (Y-Ae) is a mouse monoclonal IgG2b antibody that detects the MHC class II protein of mouse origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). MHC class II (Y-Ae) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The major histocompatibility complex (MHC) class II molecules play a crucial role in the immune response by presenting processed exogenous antigens to CD4+ T lymphocytes, which are essential for orchestrating adaptive immunity. These molecules are primarily located on the surface of antigen-presenting cells, such as dendritic cells, macrophages, and B cells, where they facilitate the recognition of foreign peptides. The ability of MHC class II molecules to bind and present 13-18 amino acid long peptides is vital for the activation of helper T cells, which in turn stimulate B cells to produce antibodies and enhance the cytotoxic activity of CD8+ T cells. The interaction between MHC class II and T cell receptors is a key event in the immune response, making anti-MHC class II antibody (Y-Ae) essential for understanding immune system dynamics and developing immunotherapies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MHC class II Antibody (Y-Ae) References:
- Autophagy Beyond Intracellular MHC Class II Antigen Presentation. | Münz, C. 2016. Trends Immunol. 37: 755-763. PMID: 27667710
- Partial MHC class II constructs as novel immunomodulatory therapy for stroke. | Benedek, G., et al. 2017. Neurochem Int. 107: 138-147. PMID: 27773790
- MHC class II expression in lung cancer. | He, Y., et al. 2017. Lung Cancer. 112: 75-80. PMID: 29191604
- Hematopoietic Cell Transplantation for MHC Class II Deficiency. | Lum, SH., et al. 2019. Front Pediatr. 7: 516. PMID: 31921728
- Spleen participation in partial MHC class II construct neuroprotection in stroke. | Brown, J., et al. 2020. CNS Neurosci Ther. 26: 663-669. PMID: 32237074
- MHC Class II Ubiquitination Regulates Dendritic Cell Function and Immunity. | Wilson, KR., et al. 2021. J Immunol. 207: 2255-2264. PMID: 34599081
- Epithelial-myeloid exchange of MHC class II constrains immunity and microbiota composition. | Stephens, WZ., et al. 2021. Cell Rep. 37: 109916. PMID: 34731608
- Molecular Determinants Regulating the Plasticity of the MHC Class II Immunopeptidome. | Santambrogio, L. 2022. Front Immunol. 13: 878271. PMID: 35651601
- MHC Class II Presentation in Autoimmunity. | Ishina, IA., et al. 2023. Cells. 12: PMID: 36672249
- PAD4 controls tumor immunity via restraining the MHC class II machinery in macrophages. | Pitter, MR., et al. 2024. Cell Rep. 43: 113942. PMID: 38489266
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MHC class II Antibody (Y-Ae) | sc-32247 | 200 µg/ml | $322.00 | |||
MHC class II Antibody (Y-Ae) AC | sc-32247 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MHC class II Antibody (Y-Ae) HRP | sc-32247 HRP | 200 µg/ml | $322.00 | |||
MHC class II Antibody (Y-Ae) FITC | sc-32247 FITC | 200 µg/ml | $336.00 | |||
MHC class II Antibody (Y-Ae) PE | sc-32247 PE | 200 µg/ml | $349.00 | |||
MHC class II Antibody (Y-Ae) Alexa Fluor® 488 | sc-32247 AF488 | 200 µg/ml | $364.00 | |||
MHC class II Antibody (Y-Ae) Alexa Fluor® 546 | sc-32247 AF546 | 200 µg/ml | $364.00 | |||
MHC class II Antibody (Y-Ae) Alexa Fluor® 594 | sc-32247 AF594 | 200 µg/ml | $364.00 | |||
MHC class II Antibody (Y-Ae) Alexa Fluor® 647 | sc-32247 AF647 | 200 µg/ml | $364.00 | |||
MHC class II Antibody (Y-Ae) Alexa Fluor® 680 | sc-32247 AF680 | 200 µg/ml | $364.00 | |||
MHC class II Antibody (Y-Ae) Alexa Fluor® 790 | sc-32247 AF790 | 200 µg/ml | $364.00 |