Ku70 Antibody (E-5): sc-17789
- Ku70 Antibody (E-5) is a mouse monoclonal IgG1 κ Ku70 antibody, cited in 93 publications, provided at 200 µg/ml
- raised against amino acids 302-609 mapping at the C-terminus of Ku70 of the Ku protein of human origin
- Ku70 Antibody (E-5) is recommended for detection of Ku70 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Ku70 Antibody (E-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Ku70 Antibody (E-5) for WB and IHC(P) applications. These reagents are now offered in bundles with Ku70 Antibody (E-5) (see ordering information below).
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Ku70 Antibody (E-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ku70 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Ku70 antibody (E-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ku70, a critical component of the Ku heterodimer alongside Ku86, is primarily located in the nucleus, where Ku70 plays a vital role in DNA repair and maintenance of genomic stability. Ku70 is essential for the non-homologous end joining (NHEJ) pathway, which repairs double-strand breaks in DNA, a common form of damage that can lead to mutations and cancer if left unrepaired. Ku70′s ability to bind to DNA ends and recruit other repair factors underscores Ku70′s importance in cellular responses to DNA damage, making Ku70 a key player in maintaining genome integrity. Ku70 has been implicated in various cellular processes, including transcriptional regulation and cell signaling, further highlighting Ku70′s multifaceted role in cellular homeostasis and response to stress.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ku70 Antibody (E-5) References:
- Identification of Ku70 Domain-Specific Interactors Using BioID2. | Abbasi, S. and Schild-Poulter, C. 2021. Cells. 10: PMID: 33799447
- Ku70 suppresses alternative end joining in G1-arrested progenitor B cells. | Liang, Z., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 34006647
- The Role of Ku70 as a Cytosolic DNA Sensor in Innate Immunity and Beyond. | Sui, H., et al. 2021. Front Cell Infect Microbiol. 11: 761983. PMID: 34746031
- Integrated analysis reveals FOXA1 and Ku70/Ku80 as targets of ivermectin in prostate cancer. | Lv, S., et al. 2022. Cell Death Dis. 13: 754. PMID: 36050295
- Signal-induced enhancer activation requires Ku70 to read topoisomerase1-DNA covalent complexes. | Tan, Y., et al. 2023. Nat Struct Mol Biol. 30: 148-158. PMID: 36747093
- Ubiquitination of Ku70 by Parkin promotes apoptosis of lens epithelial cells. | Zhang, W., et al. 2023. FEBS J. 290: 3828-3842. PMID: 37000041
- UBE4B interacts with the ITCH E3 ubiquitin ligase to induce Ku70 and c-FLIPL polyubiquitination and enhanced neuroblastoma apoptosis. | Le Clorennec, C., et al. 2023. Cell Death Dis. 14: 739. PMID: 37957138
- Ku70 senses cytosolic DNA and assembles a tumor-suppressive signalosome. | Pandey, A., et al. 2024. Sci Adv. 10: eadh3409. PMID: 38277448
- Nonclassical action of Ku70 promotes Treg-suppressive function through a FOXP3-dependent mechanism in lung adenocarcinoma. | Huang, Q., et al. 2024. J Clin Invest. 134: PMID: 39446493
- Nuclear porcupine mediates XRCC6/Ku70 S-palmitoylation in the DNA damage response. | Chen, Y., et al. 2024. Exp Hematol Oncol. 13: 109. PMID: 39497152
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ku70 Antibody (E-5) | sc-17789 | 200 µg/ml | $322.00 | |||
Ku70 Antibody (E-5): m-IgG Fc BP-HRP Bundle | sc-528315 | 200 µg Ab; 10 µg BP | $361.00 | |||
Ku70 Antibody (E-5): m-IgGκ BP-HRP Bundle | sc-520659 | 200 µg Ab, 40 µg BP | $361.00 | |||
Ku70 Antibody (E-5): m-IgG1 BP-HRP Bundle | sc-542862 | 200 µg Ab; 20 µg BP | $361.00 | |||
Ku70 Antibody (E-5) AC | sc-17789 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Ku70 Antibody (E-5) HRP | sc-17789 HRP | 200 µg/ml | $322.00 | |||
Ku70 Antibody (E-5) FITC | sc-17789 FITC | 200 µg/ml | $336.00 | |||
Ku70 Antibody (E-5) PE | sc-17789 PE | 200 µg/ml | $349.00 | |||
Ku70 Antibody (E-5) Alexa Fluor® 488 | sc-17789 AF488 | 200 µg/ml | $364.00 | |||
Ku70 Antibody (E-5) Alexa Fluor® 546 | sc-17789 AF546 | 200 µg/ml | $364.00 | |||
Ku70 Antibody (E-5) Alexa Fluor® 594 | sc-17789 AF594 | 200 µg/ml | $364.00 | |||
Ku70 Antibody (E-5) Alexa Fluor® 647 | sc-17789 AF647 | 200 µg/ml | $364.00 | |||
Ku70 Antibody (E-5) Alexa Fluor® 680 | sc-17789 AF680 | 200 µg/ml | $364.00 | |||
Ku70 Antibody (E-5) Alexa Fluor® 790 | sc-17789 AF790 | 200 µg/ml | $364.00 |