Anticorpo Histone H1 (H-2) raccomandato per il rilevamento di all histone H1 isoforms di origine mouse, rat e human in WB, IP, IF e ELISA

Anticorpo Histone H1 (H-2): sc-393358

Anticorpo Histone H1 (H-2) valutazione 4.3 di 5 di 7.
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Schede tecniche

    • Anti-Histone H1 Antibody H-2 è un monoclonale di topo IgG1 λ Histone H1 antibody, citato in 18 pubblicazioni, fornito in 200 µg/ml
    • specifico per un epitopo mappato tra gli amminoacidi 22-47 vicino al N-terminus di Histone H1 di origine human
    • Histone H1 Antibody (H-2) é raccomandato per il rilevamento di all histone H1 isoforms di origine mouse, rat e human in WB, IP, IF e ELISA
    • Histone H1 Antibody (H-2) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • reagente TransCruz per utilizzo in Gel Supershift e ChIP (sc-393358 X, 200 µg/0.1 ml)
    • Contattaci per ricevere un FREE 10 µg sample di Histone H1 (H-2): sc-393358.
    • m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for Histone H1 Antibody (H-2) for WB applications. This reagent is now offered in a bundle with Histone H1 Antibody (H-2) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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When should the TransCruz reagent sc-393358 X be used instead of the standard form sc-393358?

Richiesta da: jenni
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Risposta da: Technical Support 7
Data di pubblicazione: 2017-01-13

What secondary is best to use with Histone H1 (H-2): sc-393358?

Richiesta da: jenni
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with Histone H1 (H-2):sc-393358, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Risposta da: Technical Support 7
Data di pubblicazione: 2017-01-13
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Rated 1 di 5 di da Not good for IF Weak signal for IF in hunam HT1080 cells at 1:100 dilution.
Data di pubblicazione: 2017-09-27
Rated 5 di 5 di da Good for WB Strong single band - worked well in nuclear fractionation WB.
Data di pubblicazione: 2017-09-13
Rated 5 di 5 di da Works great for Western Blot. Used at a concentration of 1:2000 for Western blot with mouse tissues. Worked very well, clean blot and a single band. I would recommend!
Data di pubblicazione: 2017-09-01
Rated 5 di 5 di da Superb !! Great product for western blot applications; strong signal
Data di pubblicazione: 2017-01-20
Rated 5 di 5 di da good in WB This Histone H1 antibody shows specific strong bands in WB.
Data di pubblicazione: 2017-01-12
Rated 4 di 5 di da Potent and very specific antibody This monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was medium detection of the ~32-33 kDa target protein in a HeLa/Jurkat cell lysate mix, but not detected in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested or in the HeLa/Jurkat cell lysate mix.
Data di pubblicazione: 2016-11-10
Rated 5 di 5 di da Clean blot with single strong band at expected Clean blot with single strong band at expected size using A-431, LNCaP, HeLa and HL-60 whole cell lysates and Jurkat nuclear extract. -SCBT QC
Data di pubblicazione: 2014-07-05
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