Anticorpo GDF-15 (H-2) raccomandato per il rilevamento di precursor and mature GDF-15 di origine mouse, rat e human in WB, IP, IF e ELISA

Anticorpo GDF-15 (H-2): sc-515675

Anticorpo GDF-15 (H-2) valutazione 4.0 di 5 di 1.
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Schede tecniche

    • GDF-15 Antibody H-2 è un monoclonale di topo IgG2a κ, citato in 1 pubblicazioni, fornito in 200 µg/ml
    • specifico per un epitopo mappato tra gli amminoacidi 41-62 vicino al N-terminus di di origine rat
    • GDF-15 Antibody (H-2) é raccomandato per il rilevamento di precursor and mature GDF-15 di origine mouse, rat e human in WB, IP, IF e ELISA
    • GDF-15 Antibody (H-2) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Contattaci per ricevere un FREE 10 µg sample di GDF-15 (H-2): sc-515675.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GDF-15 Antibody (H-2) for WB applications. These reagents are now offered in bundles with GDF-15 Antibody (H-2) (see ordering information below).
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Please tell me the blocking peptide corresponding to this antibody (sc-515675).

Richiesta da: yuki13
Thank you for your question. We do not offer a blocking peptide for this antibody.
Risposta da: Tech Support Europe
Data di pubblicazione: 2020-07-29

I have a question. is western blot data non-reducing conditions or reducing conditions? I did WB to detect gdf15 in LNCaP by non-reducing conditions. I got 2 band : one above 40 kDa, the other around 35 kDa.

Richiesta da: Col12
Thank you for your question. Our Western blot data with this antibody is under reducing conditions. We have not tested the antibody under non-reducing conditions.
Risposta da: Technical Support
Data di pubblicazione: 2019-05-25

I have a rat cell line, can this ab detect GDF15 from rat?

Richiesta da: M76gmm
Thank you for your question. Yes, this antibody is recommended for detection of GDF15 of mouse, rat and human origin. We have Western blot data on our website in the rat cell line, RIN-m5F.
Risposta da: Technical Support
Data di pubblicazione: 2019-01-31

Has anyone any information that this Ab can detect human GDF15. According to the protein alignments in aa 41-62 the Human sequence differs by an additional 7 amino acids. Would be keen to see some experimental data if anyone has it. Thanks

Richiesta da: Satish Patel
Thank you for your question. Yes, we have human cell data on this antibody in LNCaP cells on the product page: https://www.scbt.com/scbt/product/gdf-15-antibody-h-2?requestFrom=search If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Risposta da: Tech Service
Data di pubblicazione: 2017-06-28

I need to use an antibody to detect the protein in samples of avian origin. Do you know if sc-515675: GDF-15 (H-2) mouse monoclonal antibody will work?

Richiesta da: Dr Ninau Qelp
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Risposta da: Technical Support
Data di pubblicazione: 2017-03-06
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Rated 4 di 5 di da Works well on murine cardiac samples 1h blocking in mPBST, GDF15 ab ON at 4°C, secondary ab 1 h at RT; Ab detected 2 bands: one slightly above 40 kDa, the other around 25 kDa
Data di pubblicazione: 2017-11-27
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