GAD-65 Antibody (GAD-6): sc-32270

GAD-65 Antibody (GAD-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects GAD-65 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-GAD-65 antibody (GAD-6) specifically recognizes the GAD-65 isoform, which plays a crucial role in the synthesis of gamma-aminobutyric acid (GABA), the primary inhibitory neurotransmitter in the central nervous system. GAD-65 (GAD-6) monoclonal antibody recognizes a protein predominantly localized to synaptic vesicle membranes in nerve terminals, where GAD-65 is essential for the rapid production of GABA in response to increased neuronal activity. GAD-65 ability to respond to heightened demands for GABA is vital for maintaining proper neurotransmission and preventing excitotoxicity. In contrast, GAD-67, which is also present in the brain, is responsible for maintaining basal GABA levels and is distributed throughout the cell. The interplay between these two isoforms is critical; while GAD-65 loss can lead to impaired GABA neurotransmission, GAD-67 absence is associated with severe consequences. Understanding the distinct roles and localization of these proteins is important for elucidating their contributions to neurological health and disease.

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GAD-65 Antibody (GAD-6) References:

1. Antiglutamic acid decarboxylase 65 (GAD65) antibody-associated epilepsy.  |  Daif, A., et al. 2018. Epilepsy Behav. 80: 331-336. PMID: 29433947
2. Immune-mediated epilepsy with GAD65 antibodies.  |  Li, X., et al. 2020. J Neuroimmunol. 341: 577189. PMID: 32087461
3. Low rate of glutamic acid decarboxylase 65 (GAD-65) antibodies in chronic epilepsy.  |  Tizazu, E., et al. 2020. Seizure. 80: 38-41. PMID: 32512284
4. Clinical spectrum of high-titre GAD65 antibodies.  |  Budhram, A., et al. 2021. J Neurol Neurosurg Psychiatry. 92: 645-54. PMID: 33563803
5. Postpartum State, but Not Maternal Caregiving or Level of Anxiety, Increases Medial Prefrontal Cortex GAD65 and vGAT in Female Rats.  |  Ragan, CM., et al. 2021. Front Glob Womens Health. 2: 746518. PMID: 35211693
6. A genome-wide association study in autoimmune neurological syndromes with anti-GAD65 autoantibodies.  |  Strippel, C., et al. 2023. Brain. 146: 977-990. PMID: 35348614
7. GAD65 Antibody-Associated Epilepsy.  |  Valinčiūtė, J., et al. 2023. Medicina (Kaunas). 59: PMID: 37374339
8. Cenobamate and Clobazam Combination as Personalized Medicine in Autoimmune-Associated Epilepsy With Anti-Gad65 Antibodies.  |  Serrano-Castro, PJ., et al. 2023. Neurol Neuroimmunol Neuroinflamm. 10: PMID: 37607753
9. Early Neuroaxonal Damage in Neurologic Disorders Associated With GAD65 Antibodies.  |  Eisenhut, K., et al. 2024. Neurol Neuroimmunol Neuroinflamm. 11: PMID: 37914416
10. Persistent GAD 65 antibodies in longstanding IDDM are not associated with residual beta-cell function, neuropathy or HLA-DR status.  |  Jaeger, C., et al. 1997. Horm Metab Res. 29: 510-5. PMID: 9405979

The preferred GAD-65/67 antibody is GAD-65/67 Antibody (C-9): sc-365180.