GAD-65/67 Antibody (C-9): sc-365180

GAD-65/67 Antibody (C-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects GAD-65/67 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). GAD-65/67 (C-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The GAD-65 and GAD-67 proteins, which are crucial for the synthesis of gamma-aminobutyric acid (GABA) from L-glutamic acid, play a significant role in neurotransmission within the brain. GAD-65, localized to synaptic vesicle membranes in nerve terminals, is activated during increased demand for GABA, while GAD-67, which is distributed throughout the cell, maintains basal GABA levels essential for normal neuronal function. The proper functioning of these proteins is vital, as the loss of GAD-65 can disrupt GABA neurotransmission, leading to neurological issues, whereas the absence of GAD-67 is lethal, underscoring the importance of these proteins in maintaining the balance of inhibitory signaling in the brain. Additionally, GAD-67 has two isoforms due to alternative splicing, with the predominant form being GAD-67 and a minor form known as GAD-25, which is not expressed in the brain but is found in various endocrine tissues.

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GAD-65/67 Antibody (C-9) References:

1. Neuronal activity and neurotrophic factors regulate GAD-65/67 mRNA and protein expression in organotypic cultures of rat visual cortex.  |  Patz, S., et al. 2003. Eur J Neurosci. 18: 1-12. PMID: 12859332
2. Age-related loss of the GABA synthetic enzyme glutamic acid decarboxylase in rat primary auditory cortex.  |  Ling, LL., et al. 2005. Neuroscience. 132: 1103-13. PMID: 15857714
3. Reduced GAD(65/67) immunoreactivity in the hypothalamic paraventricular nucleus in depression: a postmortem study.  |  Gao, SF., et al. 2013. J Affect Disord. 149: 422-5. PMID: 23312397
4. Different parvalbumin and GABA expression in human epileptogenic focal cortical dysplasia.  |  Medici, V., et al. 2016. Epilepsia. 57: 1109-19. PMID: 27173597
5. Pharmacological activation of dopamine D4 receptor modulates morphine-induced changes in the expression of GAD65/67 and GABAB receptors in the basal ganglia.  |  Negrete-Díaz, JV., et al. 2019. Neuropharmacology. 152: 22-29. PMID: 30682345
6. Aberrant expression of Nav1.6 in the cochlear nucleus correlates with salicylate-induced tinnitus in rats.  |  Yin, M., et al. 2020. Biochem Biophys Res Commun. 526: 786-792. PMID: 32268959
7. Single-cell multiplex qPCR evidence for sex-dimorphic glutamate decarboxylase, estrogen receptor, and 5'-AMP-activated protein kinase alpha subunit mRNA expression by ventromedial hypothalamic nucleus GABAergic neurons.  |  Ali, MH., et al. 2022. J Chem Neuroanat. 124: 102132. PMID: 35772680
8. Grape seed extract suppresses calcitonin gene-related peptide secretion and upregulates expression of GAD 65/67 and GABAB receptor in primary trigeminal ganglion cultures.  |  Antonopoulos, SR. and Durham, PL. 2022. IBRO Neurosci Rep. 13: 187-197. PMID: 36093283
9. Chrysanthemum morifolium and Its Bioactive Substance Enhanced the Sleep Quality in Rodent Models via Cl- Channel Activation.  |  Kim, M., et al. 2023. Nutrients. 15: PMID: 36986039
10. Reprogrammed human lateral ganglionic eminence precursors generate striatal neurons and restore motor function in a rat model of Huntington's disease.  |  McCaughey-Chapman, A., et al. 2024. Stem Cell Res Ther. 15: 448. PMID: 39578834

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GAD-65/67 Antibody (C-9) has 31 citations in a variety of scientific publications.

Primary antibodies like Anti-GAD-65/67 Antibody (C-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GAD-65/67 Antibody (C-9) Specifications

Alternate Product Name: GAD65/67

Product Clone Name: C-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 569-594

Epitope Target: GAD-67

Epitope Species: human

Product Reactivity/Detection: GAD-65 and GAD-67

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 31 citations in various scientific publications

Available Conjugates/Forms for GAD-65/67 Antibody (C-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin