Anticorpo EphB1 (5F10A4) raccomandato per il rilevamento di EphB1 di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA

Anticorpo EphB1 (5F10A4): sc-130054

Anticorpo EphB1 (5F10A4) valutazione 3.7 di 5 di 3.
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Schede tecniche

    • Anti-EphB1 Antibody 5F10A4 è un monoclonale di topo IgG1 (kappa light chain) EphB1 antibody fornito in 200 µg/ml
    • raised against a recombinant protein corresponding to amino acids 19-133 of EphB1 of human origin
    • raccomandato per il rilevamento di EphB1 di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA
    • Contattaci per ricevere un FREE 10 µg sample di EphB1 (5F10A4): sc-130054.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for EphB1 Antibody (5F10A4) for WB and IHC(P) applications. This reagent is now offered in a bundle with EphB1 Antibody (5F10A4) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Dear Santa Cruz I am wondering if you could address my two questions. 1) sc-9319 is described as being discontinued. This batch looks guaranteed for immunostaining so I am wondering whether you still have a stock to distribute or not.

Richiesta da: SetsuSahara
Thank you for your question. This goat polyclonal antibody, EphB1 (M-19): sc-9319, is now discontinued and no longer available for distribution. For a limited time, we are offering a free 10 µg sample of the suggested replacement for this antibody, EphB1 (5F10A4): sc-130054. Please contact our Technical Service Department if you would like to test the antibody before placing your order. Please call our European Support office toll-free at 00800 4573 8000, email [email protected], or contact us by live chat.
Risposta da: Technical Support
Data di pubblicazione: 2017-10-02

I need to use an antibody to detect the protein in samples of avian origin. Do you know if sc-130054: EphB1 (5F10A4) mouse monoclonal antibody will work?

Richiesta da: DefinitelyNotMatt
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Risposta da: Technical Support
Data di pubblicazione: 2017-03-06
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Rated 1 di 5 di da It didn't work for WB We tried this antibody (1:200 dilution as suggested) with rat brain tissue. We couldn't see any band.
Data di pubblicazione: 2020-09-01
Rated 5 di 5 di da works on cryosections I used a free sample of this antibody for IF on mouse brain sections. The signal is very clear, though an enhancement by TSA may be required.
Data di pubblicazione: 2017-10-26
Rated 5 di 5 di da Positive detection of endogenous expression Positive detection of endogenous expression of EphB1 by WB using MDA-MB-453, MCF-7 and T47-D whole cell lysates. -SCBT QC
Data di pubblicazione: 2013-03-02
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