Date published: 2025-9-9

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Anticorps VRK1 (E-3): sc-390809

5.0(2)
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Fiches techniques
  • L'Anticorps VRK1 (E-3) est un monoclonal de souris IgG1 κ, cité dans 1 publications, fourni en 200 µg/ml
  • dirigé contre les acides aminés 266-396 situés au niveau C-terminus de VRK1 d'origine human
  • recommandé pour la détection de VRK1 d'origine human par WB, IP, IF et ELISA
  • m-IgG Fc BP-HRP et 1 BP-HRP">m-IgG1 BP-HRP sont les réactifs de détection secondaire préférés pour VRK1 Antibody (E-3) for WB applications. Ces réactifs sont désormais proposés en lots avec VRK1 Antibody (E-3)(voir les informations de commande ci-dessous).

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

Human vaccina-related kinases 1 and 2 (VRK1,2) are NLS-containing, serine/threonine poxvirus-related kinases that are similar to casein kinase-1 family members. These VRK kinases phosphorylate transcription factors related to stress responses, such as p53. As an upstream regulator of p53, VRK-1 is capable of phosphorylating phosvitin, casein, histone 2b and myelin basic protein. VRK1 co-localizes with ATF2 in the nucleus and can form a stable complex. VRK1 phosphorylates ATF2 mainly on Thr 73, stabilizing the ATF2 protein and increasing its intracellular level. VRK1 phosphorylates human p53 in Thr18 and disrupts p53-Mdm2 interaction in vitro. VRK1 phosphorylates c-Jun in Ser 63 and Ser 73 in vitro (the same residues targeted by the N-terminal kinase of c-Jun (JNK)), and activates c-Jun dependent transcription.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps VRK1 (E-3)

sc-390809
200 µg/ml
$316.00

VRK1 (E-3): m-IgG Fc BP-HRP Kit

sc-540858
200 µg Ab; 10 µg BP
$354.00

VRK1 (E-3): m-IgG1 BP-HRP Kit

sc-542421
200 µg Ab; 20 µg BP
$354.00

What is the recommended starting dilution for Western blot for sc-390809: VRK1 (E-3) monoclonal antibody?

Posée par: TinTin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Répondue par: Technical Support
Date de publication: 2017-03-22
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Rated 5 de 5 de par Produced positive Western Blot data of VRK1Produced positive Western Blot data of VRK1 expression in non-transfected and human VRK1 transfected 293T whole cell lysates. -SCBT QC
Date de publication: 2015-05-04
Rated 5 de 5 de par Great Western Blot data of VRK1 expressionGreat Western Blot data of VRK1 expression in HeLa whole cell lysate. -SCBT QC
Date de publication: 2014-11-15
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Anticorps VRK1 (E-3) est évalué 5.0 de 5 de 2.
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