TDP2 Antibody (E-6): sc-515179

TDP2 Antibody (E-6) is a mouse monoclonal IgG1 antibody that detects TDP2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TDP2, also known as Ets-1-associated protein II, plays a crucial role in the regulation of Ets-1 transcriptional activity, acting as a negative modulator. This interaction helps maintain the balance of gene expression regulated by Ets-1, which is essential for various cellular processes, including differentiation and proliferation. TDP2 is predominantly localized in the nucleus, where TDP2 can effectively interact with Ets-1 and influence its function. TDP2′s ability to modulate Ets-1 activity is particularly important in the context of cellular responses to stress and DNA damage, as Ets-1 is involved in the transcriptional regulation of genes that respond to these stimuli. Anti-TDP2 antibody (E-6) enables researchers to gain insights into the intricate regulatory mechanisms governing Ets-1 activity and its implications in various biological processes.

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TDP2 Antibody (E-6) References:

1. TDP2/TTRAP is the major 5'-tyrosyl DNA phosphodiesterase activity in vertebrate cells and is critical for cellular resistance to topoisomerase II-induced DNA damage.  |  Zeng, Z., et al. 2011. J Biol Chem. 286: 403-9. PMID: 21030584
2. TDP2-dependent non-homologous end-joining protects against topoisomerase II-induced DNA breaks and genome instability in cells and in vivo.  |  Gómez-Herreros, F., et al. 2013. PLoS Genet. 9: e1003226. PMID: 23505375
3. Proteolytic degradation of topoisomerase II (Top2) enables the processing of Top2·DNA and Top2·RNA covalent complexes by tyrosyl-DNA-phosphodiesterase 2 (TDP2).  |  Gao, R., et al. 2014. J Biol Chem. 289: 17960-9. PMID: 24808172
4. Novel TDP2-ubiquitin interactions and their importance for the repair of topoisomerase II-mediated DNA damage.  |  Rao, T., et al. 2016. Nucleic Acids Res. 44: 10201-10215. PMID: 27543075
5. Synthesis and structure-activity relationship of furoquinolinediones as inhibitors of Tyrosyl-DNA phosphodiesterase 2 (TDP2).  |  Yu, LM., et al. 2018. Eur J Med Chem. 151: 777-796. PMID: 29677635
6. Effect of TDP2 on the Level of TOP2-DNA Complexes and SUMOylated TOP2-DNA Complexes.  |  Lee, KC., et al. 2018. Int J Mol Sci. 19: PMID: 30011940
7. Confirming TDP2 mutation in spinocerebellar ataxia autosomal recessive 23 (SCAR23).  |  Zagnoli-Vieira, G., et al. 2018. Neurol Genet. 4: e262. PMID: 30109272
8. Triazolopyrimidine and triazolopyridine scaffolds as TDP2 inhibitors.  |  Ribeiro, CJA., et al. 2019. Bioorg Med Chem Lett. 29: 257-261. PMID: 30522956
9. The synthesis of furoquinolinedione and isoxazoloquinolinedione derivatives as selective Tyrosyl-DNA phosphodiesterase 2 (TDP2) inhibitors.  |  Yang, H., et al. 2021. Bioorg Chem. 111: 104881. PMID: 33839584
10. A novel non-sense mutation in TDP2 causes spinocerebellar ataxia autosomal recessive 23 accompanied by bilateral upward gaze; report of a case and review of the literature.  |  Zoghi, S., et al. 2021. Eur J Med Genet. 64: 104348. PMID: 34606976

The preferred TDP2 antibody is TDP2 Antibody (H-6): sc-377280.