Spot 14 Antibody (F-7): sc-137178
- Spot 14 Antibody (F-7) is a mouse monoclonal IgG1 κ Spot 14 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 90-146 mapping at the C-terminus of Spot 14 of human origin
- Spot 14 Antibody (F-7) is recommended for detection of Spot 14 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Spot 14 Antibody (F-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Spot 14 Antibody (F-7) for WB applications. These reagents are now offered in bundles with Spot 14 Antibody (F-7) (see ordering information below).
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Spot 14 Antibody (F-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Spot 14 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Spot 14 (F-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Spot 14, also known as S14 or thyroid hormone responsive protein (THRSP), is a small acidic protein primarily located in the nucleus, where Spot 14 plays a crucial role in regulating lipid metabolism. Spot 14 is predominantly expressed in tissues involved in triglyceride synthesis, such as liver, mammary glands, and adipose tissues. Spot 14 (F-7) monoclonal antibody is essential for studying growth and differentiation, as Spot 14 functions as a transcriptional regulator for genes encoding proteins involved in lipogenesis. Spot 14 expression is activated by various lipogenic stimuli, including thyroid hormone, dietary carbohydrates, insulin, and glucose, while being downregulated by catecholamines and glucagon. Spot 14 expression exhibits oscillation in accordance with circadian clock, and Spot 14 knockdown impairs lipid synthesis and induces apoptosis. In many breast cancers, Spot 14 is overexpressed, suggesting a role in promoting cell growth and survival, making Spot 14 a significant target for research in cancer biology and metabolic disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Spot 14 Antibody (F-7) References:
- Spot 14 protein interacts and co-operates with chicken ovalbumin upstream promoter-transcription factor 1 in the transcription of the L-type pyruvate kinase gene through a specificity protein 1 (Sp1) binding site. | Compe, E., et al. 2001. Biochem J. 358: 175-83. PMID: 11485565
- The spot 14 protein inhibits growth and induces differentiation and cell death of human MCF-7 breast cancer cells. | Sanchez-Rodriguez, J., et al. 2005. Biochem J. 390: 57-65. PMID: 15819613
- Expression of 'Spot 14' (THRSP) predicts disease free survival in invasive breast cancer: immunohistochemical analysis of a new molecular marker. | Wells, WA., et al. 2006. Breast Cancer Res Treat. 98: 231-40. PMID: 16552628
- S14: insights from knockout mice. | LaFave, LT., et al. 2006. Endocrinology. 147: 4044-7. PMID: 16809440
- Spot 14: A marker of aggressive breast cancer and a potential therapeutic target. | Kinlaw, WB., et al. 2006. Endocrinology. 147: 4048-55. PMID: 16809441
- Multifactorial regulation of daily rhythms in expression of the metabolically responsive gene spot14 in the mouse liver. | Ishihara, A., et al. 2007. J Biol Rhythms. 22: 324-34. PMID: 17660449
- Cloning and initial characterization of human and mouse Spot 14 genes. | Grillasca, JP., et al. 1997. FEBS Lett. 401: 38-42. PMID: 9003802
- The 'Spot 14' gene resides on the telomeric end of the 11q13 amplicon and is expressed in lipogenic breast cancers: implications for control of tumor metabolism. | Moncur, JT., et al. 1998. Proc Natl Acad Sci U S A. 95: 6989-94. PMID: 9618526
- 'Spot 14' protein: a metabolic integrator in normal and neoplastic cells. | Cunningham, BA., et al. 1998. Thyroid. 8: 815-25. PMID: 9777755
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Spot 14 Antibody (F-7) | sc-137178 | 200 µg/ml | $322.00 | |||
Spot 14 Antibody (F-7): m-IgG Fc BP-HRP Bundle | sc-527126 | 200 µg Ab; 10 µg BP | $361.00 | |||
Spot 14 Antibody (F-7): m-IgG1 BP-HRP Bundle | sc-532499 | 200 µg Ab; 20 µg BP | $361.00 | |||
Spot 14 Antibody (F-7) AC | sc-137178 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Spot 14 Antibody (F-7) HRP | sc-137178 HRP | 200 µg/ml | $322.00 | |||
Spot 14 Antibody (F-7) FITC | sc-137178 FITC | 200 µg/ml | $336.00 | |||
Spot 14 Antibody (F-7) PE | sc-137178 PE | 200 µg/ml | $349.00 | |||
Spot 14 Antibody (F-7) Alexa Fluor® 488 | sc-137178 AF488 | 200 µg/ml | $364.00 | |||
Spot 14 Antibody (F-7) Alexa Fluor® 546 | sc-137178 AF546 | 200 µg/ml | $364.00 | |||
Spot 14 Antibody (F-7) Alexa Fluor® 594 | sc-137178 AF594 | 200 µg/ml | $364.00 | |||
Spot 14 Antibody (F-7) Alexa Fluor® 647 | sc-137178 AF647 | 200 µg/ml | $364.00 | |||
Spot 14 Antibody (F-7) Alexa Fluor® 680 | sc-137178 AF680 | 200 µg/ml | $364.00 | |||
Spot 14 Antibody (F-7) Alexa Fluor® 790 | sc-137178 AF790 | 200 µg/ml | $364.00 |