Date published: 2025-9-7

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Anticorps SAP 61 (A-5): sc-376266

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Fiches techniques
  • L'Anticorps SAP 61 (A-5) est un monoclonal de souris IgG2b (chaîne légère kappa) fourni en 200 µg/ml
  • dirigé contre les acides aminés 1-270 situés au niveau N-terminus de SAP 61 d'origine human
  • recommandé pour la détection de SAP 61 d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA; également réactif avec d'autres espèces, dont et equine, canine, bovine and porcine
  • m-IgG2b BP-HRP est le réactif secondaire recommandé pour la détection de l'anticorps SAP 61 (A-5) pour des applications de WB and IHC(P). Ce réactif est maintenant proposé en kit avec l'anticorps SAP 61 (A-5) (voir Informations pour la commande ci-dessous).

    ACCÈS RAPIDE AUX LIENS

    VOIR ÉGALEMENT...

    SAP 61, also known as SF3A3 (splicing factor 3A subunit 3), PRP9, PRPF9 or SF3a60, is a 501 amino acid protein that contains one matrin-type zinc finger and belongs to the SF3A3 family. Localized to the nucleus, SAP 61 is a subunit of the SF3A splicing factor, a heterotrimeric complex comprised of three subunits that act in tandem to mediate the binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. The SF3A complex is necessary for the conversion of 15S U2 snRNP into the active 17S protein that performs directly in pre-mRNA splicing events. Functioning as the third subunit of the complex, SAP 61 interacts with subunit 1 (SAP 114) via its N-terminus, while simultaneously binding to 15S U2 snRNP via its zinc finger domain. As is the case for all SF3A subunits, SAP 61 is essential for prespliceosome assembly and cell viability. In addition, a pseudogene exists for SAP 61 on chromosome 20.

    Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

    Informations pour la commande

    Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

    Anticorps SAP 61 (A-5)

    sc-376266
    200 µg/ml
    $316.00

    SAP 61 (A-5): m-IgG2b BP-HRP Kit

    sc-548591
    200 µg Ab; 10 µg BP
    $354.00

    Can SAP 61 (A-5): sc-376266 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

    Posée par: TinTin
    Thank you for your question. Yes, SAP 61 (A-5): sc-376266 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
    Répondue par: Technical Support
    Date de publication: 2017-02-28
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    Rated 5 de 5 de par Robust band observed in WB in ARobust band observed in WB in A-431 whole cell lysate. -SCBT QC
    Date de publication: 2015-07-12
    Rated 5 de 5 de par Produced exceptional immunofluorescence nuclearProduced exceptional immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
    Date de publication: 2015-01-04
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    Anticorps SAP 61 (A-5) est évalué 5.0 de 5 de 2.
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