SAF-B2 Antibody (C-5): sc-514963
- SAF-B2 Antibody (C-5) is a mouse monoclonal IgG2b κ SAF-B2 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 898-922 near the C-terminus of SAF-B2 of human origin
- SAF-B2 Antibody (C-5) is recommended for detection of SAF-B2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-SAF-B2 Antibody (C-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SAF-B2 Antibody (C-5) for WB applications. These reagents are now offered in bundles with SAF-B2 Antibody (C-5) (see ordering information below).
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SAF-B2 Antibody (C-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects SAF-B2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SAF-B2 (C-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SAF-B2, also known as scaffold attachment factor B2, plays a crucial role in gene expression regulation by binding to matrix- or scaffold-associating regions (MARs or SARs) on DNA, which is essential for chromatin organization and transcription regulation. SAF-B2 interacts with RNA polymerase II and serine-/arginine-rich RNA processing factors, facilitating pre-mRNA processing and transport. SAF-B2 is expressed in various breast cancer cell lines, where SAF-B2 may influence cellular proliferation and division by interacting with the estrogen receptor. Unlike SAF-B, which is exclusively nuclear, SAF-B2 is localized in both the cytoplasm and nucleus, highlighting SAF-B2′s versatile role in cellular processes. SAF-B2′s ability to shuttle between these compartments underscores SAF-B2′s importance in dynamic regulation of gene expression and cellular response to hormonal signals.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SAF-B2 Antibody (C-5) References:
- Estrogen-mediated downregulation of CD24 in breast cancer cells. | Kaipparettu, BA., et al. 2008. Int J Cancer. 123: 66-72. PMID: 18404683
- SAFB1's multiple functions in biological control-lots still to be done! | Garee, JP. and Oesterreich, S. 2010. J Cell Biochem. 109: 312-9. PMID: 20014070
- Quantitative proteomic identification of the BRCA1 ubiquitination substrates. | Song, M., et al. 2011. J Proteome Res. 10: 5191-8. PMID: 21950761
- Unravelling the RNA-Binding Properties of SAFB Proteins in Breast Cancer Cells. | Hong, E., et al. 2015. Biomed Res Int. 2015: 395816. PMID: 26273616
- Intranuclear Mobility of Estrogen Receptor: Implication for Transcriptional Regulation. | Matsuda, KI., et al. 2018. Acta Histochem Cytochem. 51: 129-136. PMID: 30279614
- Scaffold attachment factor B: distribution and interaction with ERα in the rat brain. | Hashimoto, T., et al. 2020. Histochem Cell Biol. 153: 323-338. PMID: 32086573
- Large scale RNA-binding proteins/LncRNAs interaction analysis to uncover lncRNA nuclear localization mechanisms. | Huang, Y., et al. 2021. Brief Bioinform. 22: PMID: 34056657
- Insight into the Structural Basis for Dual Nucleic Acid-Recognition by the Scaffold Attachment Factor B2 Protein. | Korn, SM., et al. 2023. Int J Mol Sci. 24: PMID: 36834708
- SAFB associates with nascent RNAs and can promote gene expression in mouse embryonic stem cells. | Cherney, RE., et al. 2023. RNA. 29: 1535-1556. PMID: 37468167
- Quantitative phosphoproteomics reveals molecular pathway network alterations in human early-stage primary hepatic carcinomas: potential for 3P medical approach. | Zhang, Y., et al. 2023. EPMA J. 14: 477-502. PMID: 37605650
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SAF-B2 Antibody (C-5) | sc-514963 | 200 µg/ml | $322.00 | |||
SAF-B2 Antibody (C-5): m-IgGκ BP-HRP Bundle | sc-524887 | 200 µg Ab, 40 µg BP | $361.00 | |||
SAF-B2 Antibody (C-5): m-IgG2b BP-HRP Bundle | sc-549194 | 200 µg Ab; 10 µg BP | $361.00 | |||
SAF-B2 Antibody (C-5) AC | sc-514963 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SAF-B2 Antibody (C-5) HRP | sc-514963 HRP | 200 µg/ml | $322.00 | |||
SAF-B2 Antibody (C-5) FITC | sc-514963 FITC | 200 µg/ml | $336.00 | |||
SAF-B2 Antibody (C-5) PE | sc-514963 PE | 200 µg/ml | $349.00 | |||
SAF-B2 Antibody (C-5) Alexa Fluor® 488 | sc-514963 AF488 | 200 µg/ml | $364.00 | |||
SAF-B2 Antibody (C-5) Alexa Fluor® 546 | sc-514963 AF546 | 200 µg/ml | $364.00 | |||
SAF-B2 Antibody (C-5) Alexa Fluor® 594 | sc-514963 AF594 | 200 µg/ml | $364.00 | |||
SAF-B2 Antibody (C-5) Alexa Fluor® 647 | sc-514963 AF647 | 200 µg/ml | $364.00 | |||
SAF-B2 Antibody (C-5) Alexa Fluor® 680 | sc-514963 AF680 | 200 µg/ml | $364.00 | |||
SAF-B2 Antibody (C-5) Alexa Fluor® 790 | sc-514963 AF790 | 200 µg/ml | $364.00 | |||
SAF-B2 (C-5) Neutralizing Peptide | sc-514963 P | 100 µg/0.5 ml | $69.00 |