SAF-B2 Antibody (E-2): sc-393689

SAF-B2 Antibody (E-2) is a mouse monoclonal IgM antibody that detects SAF-B2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SAF-B2 Antibody (E-2) is available in a non-conjugated form, providing flexibility for various experimental needs. SAF-B2, also known as scaffold attachment factor B2, plays a crucial role in the organization of the nuclear matrix and is involved in the regulation of gene expression. SAF-B2 binds to matrix- or scaffold-associating regions (MARs or SARs) on DNA, facilitating interactions with RNA polymerase II and serine-/arginine-rich RNA processing factors, which are essential for pre-mRNA processing and transport. SAF-B2 is expressed in several breast cancer cell lines, where SAF-B2 may influence cellular proliferation and division by interacting with the estrogen receptor. Unlike SAF-B, which is exclusively localized in the nucleus, SAF-B2 is found in both the cytoplasm and nucleus, highlighting SAF-B2′s versatile role in cellular processes. SAF-B2′s ability to shuttle between these compartments may be significant for its function in gene regulation and cancer biology, making anti-SAF-B2 antibody (E-2) a valuable tool for researchers studying these pathways.

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SAF-B2 Antibody (E-2) References:

1. A novel hnRNP protein (HAP/SAF-B) enters a subset of hnRNP complexes and relocates in nuclear granules in response to heat shock.  |  Weighardt, F., et al. 1999. J Cell Sci. 112 (Pt 10): 1465-76. PMID: 10212141
2. Tamoxifen-bound estrogen receptor (ER) strongly interacts with the nuclear matrix protein HET/SAF-B, a novel inhibitor of ER-mediated transactivation.  |  Oesterreich, S., et al. 2000. Mol Endocrinol. 14: 369-81. PMID: 10707955
3. A nuclear matrix-associated factor, SAF-B, interacts with specific isoforms of AUF1/hnRNP D.  |  Arao, Y., et al. 2000. Arch Biochem Biophys. 380: 228-36. PMID: 10933876
4. HET/SAF-B overexpression causes growth arrest and multinuclearity and is associated with aneuploidy in human breast cancer.  |  Townson, SM., et al. 2000. Clin Cancer Res. 6: 3788-96. PMID: 10999774
5. Structure and dynamics of hnRNP-labelled nuclear bodies induced by stress treatments.  |  Chiodi, I., et al. 2000. J Cell Sci. 113 (Pt 22): 4043-53. PMID: 11058091
6. SAFB2, a new scaffold attachment factor homolog and estrogen receptor corepressor.  |  Townson, SM., et al. 2003. J Biol Chem. 278: 20059-68. PMID: 12660241
7. No germline mutations in supposed tumour suppressor genes SAFB1 and SAFB2 in familial breast cancer with linkage to 19p.  |  Bergman, A., et al. 2008. BMC Med Genet. 9: 108. PMID: 19077293
8. Low SAFB levels are associated with worse outcome in breast cancer patients.  |  Hammerich-Hille, S., et al. 2010. Breast Cancer Res Treat. 121: 503-9. PMID: 19137425
9. SAFB1 mediates repression of immune regulators and apoptotic genes in breast cancer cells.  |  Hammerich-Hille, S., et al. 2010. J Biol Chem. 285: 3608-3616. PMID: 19901029
10. Identification and characterisation of a novel human RNA-binding protein.  |  Badolato, J., et al. 1995. Gene. 166: 323-7. PMID: 8543184
11. Novel nuclear matrix protein HET binds to and influences activity of the HSP27 promoter in human breast cancer cells.  |  Oesterreich, S., et al. 1997. J Cell Biochem. 67: 275-86. PMID: 9328833
12. SAF-B protein couples transcription and pre-mRNA splicing to SAR/MAR elements.  |  Nayler, O., et al. 1998. Nucleic Acids Res. 26: 3542-9. PMID: 9671816

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SAF-B2 Antibody (E-2) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-SAF-B2 Antibody (E-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SAF-B2 Antibody (E-2) Specifications

Alternate Product Name: SAF-B2

Product Clone Name: E-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 101-207

Epitope Target: SAF-B2

Epitope Species: mouse

Product Reactivity/Detection: SAF-B2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for SAF-B2 Antibody (E-2): N/A