Anticorps p21 Waf1/Cip1/CDKN1A (F-5) recommandé pour la détection de p21 Waf1/Cip1 d'origine mouse, rat et human par WB, IP, IF, IHC(P) et FCM

Anticorps p21 Waf1/Cip1/CDKN1A (F-5): sc-6246

Anticorps p21 Waf1/Cip1/CDKN1A (F-5) est évalué 4.4 de 5 de 91.
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Fiches techniques

    • Anti-L'anticorps p21 Waf1/Cip1/CDKN1A F-5 est un monoclonal IgG2b κ L'Anticorps p21 Waf1/Cip1/CDKN1A, cité dans 1.589 publications, fourni en 200 µg/ml
    • dirigé contre les acides aminés 1-159 représentant la protéine totale p21 Waf1/Cip1 de mouse
    • Anti-L'Anticorps p21 Waf1/Cip1/CDKN1A (F-5) est recommandé pour la détection de p21 Waf1/Cip1 d'origine mouse, rat et human par WB, IP, IF, IHC(P) et FCM
    • Anti-L'Anticorps p21 Waf1/Cip1/CDKN1A (F-5) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • aussi disponible conjugué à la biotine pour WB, IHC(P) et ELISA; et soit au TRITC ou Alexa Fluor® 405 pour IF, IHC(P) et FCM
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de p21 Waf1/Cip1/CDKN1A (F-5): sc-6246.
    • m-IgG Fc BP-HRP et m-IgG2b BP-HRP sont les réactifs secondaires recommandés pour la détection de l'anticorps p21 Waf1/Cip1/CDKN1A (F-5) pour des applications de WB and IHC(P). Ces réactifs sont maintenant proposés en kit avec l'anticorps p21 Waf1/Cip1/CDKN1A (F-5) (voir Informations pour la commande ci-dessous).
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What is the recommended antigen retrieval protocol for IHC-P using this antibody?

Posée par: nmurao
Thank you for your question. You can find our recommended antigen retrieval method in our standard protocol which is available under this link: https://www.scbt.com/de/resources/protocols/immunoperoxidase-staining
Répondue par: Tech Support Europe
Date de publication: 2020-09-28

I'm having issues with the detection of p21 wih this antibody in A549 cells treated with nutlin or etoposide. Can you recommend western blot conditions for this cell line and protein? I'm using 1:200 dilution in TBST milk. Thanks

Posée par: eabd2
Thank you for your question. We are sorry to hear that this antibody isn't performing as expected. We want you to be successful in your research! Please contact our Technical Service Department by phone, e-mail or live chat. We are happy to help you troubleshoot the antibody.
Répondue par: Tech Service
Date de publication: 2019-11-14

What is the recommended blocking solution for p21 ab in WB?

Posée par: Sonia S
Tack för din fråga! We recommend blocking in 5% milk and TBS-T for Western blot with our p21 antibodies.
Répondue par: Tech Service
Date de publication: 2021-02-04

I used this p21 antibody for Western blotting of both cell sample and tissue sample. For cell sample, the band is at 21 kD; but for tissue, the band is at around 25 kD. Is that normal? What is the probable explanation of it?

Posée par: Liuershiba
Thank you for your question. The difference in observed molecular weight in your samples could be due to post-translational modification of the p21 protein in the tissue that is not present in the cells. However, inclusion of proper positive and negative controls is always recommended.
Répondue par: Technical Support
Date de publication: 2019-06-16

If I am using this antibody to stain P21 positive cells in the brain via immunofluorescence, what secondary antibody should I use?

Posée par: khanson
Thank you for your question. p21 Antibody (F-5) is a mouse monoclonal IgG2b, so we would recommend using a secondary antibody that is anti-mouse IgG, or anti-mouse IgG2b for detection. One example for immunofluorescence is our mouse IgG kappa binding protein (m-IgGκ BP) conjugated to CruzFluor™ 488 (CFL 488), m-IgGκ BP-CFL 488: sc-516176.
Répondue par: Technical Support
Date de publication: 2019-04-20

p21 Antibody (F-5): sc-6246  Is this antibody work for Rhesus monkey?

Posée par: Fei38750
Thank you for your question. We are aware of several product citations using this antibody in monkey, including Vero cells. However, nothing specifically in Rhesus macaque.
Répondue par: Technical Support
Date de publication: 2019-04-13

I've recently got the sample F-15 antibody sample and ran the WB using C2C12, mouse myoblast cells. But all the time, I can see the major bands between 15 and 20kD. I used 15% gel. Is it correct size? The size of this protein is not 21kDa?

Posée par: Aiden
Thank you for your question. As you mentioned the expected molecular weight of p21 is 21 kDa, and that is what is expected when using this mouse monoclonal, sc-6246, p21 (F-5). We would recommend including a secondary antibody control for your Western blot, where you incubate the membrane without primary antibody. That would identify any non-specific bands that are the result of your secondary antibody alone. A band observed below 15 kDa would be a concern, but since it is normal to see a variation of +/- 5Kda in a western (could be marker differences, gel uneveness, etc), I believe the band you are seeing is p21. Please contact our Technical Service Department if you would like to discuss this further!
Répondue par: Tech Service
Date de publication: 2021-06-03

Is it possible to provide the MSDS fiche for this product?

Posée par: BDCC
Thank you for your question. We do not provide MSDSs for our antibody products, but please contact our European office and they will be glad to help get you the information you need. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Répondue par: Tech Service
Date de publication: 2017-06-09
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Rated 4 de 5 de par Works well! We blotted several times with this antibody and it works perfectly!
Date de publication: 2021-06-16
Rated 5 de 5 de par 功能很出色! 我一个月前购买了此产品,并为此感到高兴,下次有机会还会来购买!另外圣克鲁兹的产品价格便宜实惠,实验效果好,实在是太棒了!
Date de publication: 2020-11-20
Rated 3 de 5 de par è cross reattivo nel cane ma non nel cavallo non è cross reattivo nella specie equina, è comunque cross reattivo nel cane
Date de publication: 2020-10-07
Rated 3 de 5 de par For WB We got a free sample of this antibody for WB. This antibody could detect p21 of p21 transfected 293T cell lysate easily. We will use this antibody for WB from now on.
Date de publication: 2019-07-17
Rated 2 de 5 de par Immunofluorescence on mouse endothelial cells I tested the antibody in immunofluorescence and I obtained nuclear staining of only few cells.
Date de publication: 2019-04-28
Rated 5 de 5 de par Good Ab for IF This antibody is good for immunofluorescent staining (1:50 in 1%BSA in PBS). Highly recommended!
Date de publication: 2018-11-13
Rated 5 de 5 de par Very good anti-p21 in western blot Very good anti-p21 in western blot at 1/1000 dilution in TBS-tween 5% milk
Date de publication: 2018-10-03
Rated 5 de 5 de par good product Good antibody with clean background for Western Blot.
Date de publication: 2018-09-11
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