IKK gamma Antibody (72C627): sc-56919

IKKγ Antibody (72C627) is a mouse monoclonal IgG1 antibody engineered to detect IKKγ, also known as NEMO (NF-κB Essential Modulator), in human samples using western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). IKKγ is a pivotal regulatory subunit of the IKK complex, characterized by its intricate structure that includes zinc finger domains, a coiled-coil region, and a ubiquitin-binding domain, which are essential for its role in facilitating protein-protein interactions and maintaining the structural integrity of the complex. This structural configuration is crucial for the activation of the NF-κB signaling pathway, as IKKγ mediates the phosphorylation and subsequent degradation of IκB inhibitors, allowing NF-κB to translocate to the nucleus and regulate genes involved in immune responses, inflammation, and cell survival. Post-translational modifications, particularly ubiquitination, play a significant role in regulating IKKγ′s activity and interactions with other proteins such as IKKα and IKKβ, thereby controlling the intensity and duration of the NF-κB response. IKKγ interacts with various proteins, including ubiquitin ligases and kinases, which are essential for its function in signal transduction and cellular responses to proinflammatory cytokines. By targeting the full-length native IKKγ, anti-IKKγ antibody (72C627) ensures accurate detection and analysis of this protein′s interactions and modifications, providing researchers with a reliable tool to investigate the molecular mechanisms underlying diseases like cancer, autoimmune disorders, and chronic inflammation.

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IKK gamma Antibody (72C627) References:

1. NF-kappaB activation: Nondegradative ubiquitination implicates NEMO.  |  Israël, A. 2006. Trends Immunol. 27: 395-7. PMID: 16857427
2. Regulation and function of IKK and IKK-related kinases.  |  Häcker, H. and Karin, M. 2006. Sci STKE. 2006: re13. PMID: 17047224
3. Protein-protein interactions involving IKKgamma (NEMO) that promote the activation of NF-kappaB.  |  Shifera, AS. 2010. J Cell Physiol. 223: 558-61. PMID: 20301198
4. The zinc finger domain of IKKγ (NEMO) protein in health and disease.  |  Shifera, AS. 2010. J Cell Mol Med. 14: 2404-14. PMID: 20345847
5. Proteins that bind to IKKgamma (NEMO) and down-regulate the activation of NF-kappaB.  |  Shifera, AS. 2010. Biochem Biophys Res Commun. 396: 585-9. PMID: 20457134
6. Recent advances in the structure-based and ligand-based design of IKKβ inhibitors as anti-inflammation and anti-cancer agents.  |  Huang, JJ., et al. 2014. Curr Med Chem. 21: 3893-917. PMID: 25139725
7. EDA-ID and IP, two faces of the same coin: how the same IKBKG/NEMO mutation affecting the NF-κB pathway can cause immunodeficiency and/or inflammation.  |  Fusco, F., et al. 2015. Int Rev Immunol. 34: 445-59. PMID: 26269396
8. Peptidyl arginine deiminase-4 exacerbates ischemic AKI by finding NEMO.  |  Rabadi, MM., et al. 2019. Am J Physiol Renal Physiol. 316: F1180-F1190. PMID: 30943066
9. Cardiomyocyte-specific regression of nitrosative stress-mediated S-Nitrosylation of IKKγ alleviates pathological cardiac hypertrophy.  |  Datta Chaudhuri, R., et al. 2022. Cell Signal. 98: 110403. PMID: 35835332
10. NEMO- and RelA-dependent NF-κB signaling promotes small cell lung cancer.  |  Koerner, L., et al. 2023. Cell Death Differ. 30: 938-951. PMID: 36653597

The preferred IKKγ antibody is IKKγ Antibody (B-3): sc-8032.