Anticorps IKK gamma (F-10) recommandé pour la détection de IKKγ d'origine mouse, rat et human par WB, IP et IF

Anticorps IKKγ (F-10): sc-166398

Anticorps IKKγ (F-10) est évalué 4.4 de 5 de 5.
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Fiches techniques

    • Anti-L'anticorps IKK gamma F-10 est un monoclonal IgG1 κ L'Anticorps IKKγ, cité dans 3 publications, fourni en 200 µg/ml
    • dirigé contre la protéine totale IKKΓ de human
    • Anti-L'Anticorps IKK gamma (F-10) est recommandé pour la détection de IKKγ d'origine mouse, rat et human par WB, IP et IF
    • Anti-L'Anticorps IKK gamma (F-10) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de IKKγ (F-10): sc-166398.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for IKKγ Antibody (F-10) for WB applications. This reagent is now offered in a bundle with IKKγ Antibody (F-10) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Can IKKγ (F-10): sc-166398 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Posée par: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, IKKγ (F-10): sc-166398 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Répondue par: Technical Support
Date de publication: 2017-02-24
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Rated 5 de 5 de par Good antibody for detecting endogenous NEMO Excellent antibody for detecting endogenous NEMO. It is also good for IP.
Date de publication: 2017-09-22
Rated 2 de 5 de par MW band wrong size. I previously used a Santa Cruz anti-IKKg antibody and liked it very much. As it was discontinued, I tried this one instead. There is a faint band that is almost the right size (a bit low), and several strong bands of completely the wrong size.
Date de publication: 2017-03-07
Rated 5 de 5 de par Works well antibody works well in WB of lysis of MEFs/BMDM cells
Date de publication: 2016-12-02
Rated 5 de 5 de par Highly potent and very specific antibody This monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was very strong detection of the ~48 kDa target protein in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested.
Date de publication: 2016-11-10
Rated 5 de 5 de par Fantastic WB data in mouse and human transfected Fantastic WB data in mouse and human transfected lysates. -SCBT QC
Date de publication: 2013-02-02
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