gp91phox/CYBB/NOX2 Antibody (CL5): sc-130549

gp91-phox Antibody (CL5) is a mouse monoclonal IgG1 antibody that detects gp91-phox in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. gp91-phox, a critical component of the NADPH oxidase complex, plays a vital role in generating reactive oxygen species, which are essential for various cellular processes, including host defense and signaling pathways. gp91-phox is primarily located in the plasma membrane of phagocytes, such as neutrophils and eosinophils, where gp91-phox functions as a surface oxygen sensor that triggers cellular responses to hypoxia. Proper localization of gp91-phox is crucial, as gp91-phox allows for rapid generation of superoxide in response to inflammatory stimuli, thereby facilitating immune response. gp91-phox interacts with other cytosolic proteins, such as p47-phox and p67-phox, which are necessary for assembly and activation of the NADPH oxidase complex. Unique expression patterns of gp91-phox in immune cells and upregulation in vascular smooth muscle cells under certain conditions highlight gp91-phox importance in both innate immunity and vascular biology. anti-gp91-phox antibody (CL5) serves as an invaluable tool for researchers studying oxidative stress, inflammation, and related signaling pathways.

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gp91phox/CYBB/NOX2 Antibody (CL5) References:

1. Eosinophil-specific regulation of gp91(phox) gene expression by transcription factors GATA-1 and GATA-2.  |  Yang, D., et al. 2000. J Biol Chem. 275: 9425-32. PMID: 10734088
2. Induction of gp91-phox, a component of the phagocyte NADPH oxidase, in microglial cells during central nervous system inflammation.  |  Green, SP., et al. 2001. J Cereb Blood Flow Metab. 21: 374-84. PMID: 11323523
3. Pivotal role of a gp91(phox)-containing NADPH oxidase in angiotensin II-induced cardiac hypertrophy in mice.  |  Bendall, JK., et al. 2002. Circulation. 105: 293-6. PMID: 11804982
4. Hereditary deficiency of gp91(phox) is associated with enhanced arterial dilatation: results of a multicenter study.  |  Violi, F., et al. 2009. Circulation. 120: 1616-22. PMID: 19805647
5. GCN5 regulates the superoxide-generating system in leukocytes via controlling gp91-phox gene expression.  |  Kikuchi, H., et al. 2011. J Immunol. 186: 3015-22. PMID: 21278346
6. Equol is neuroprotective during focal cerebral ischemia and reperfusion that involves p-Src and gp91(phox).  |  Yu, W., et al. 2014. Curr Neurovasc Res. 11: 367-77. PMID: 25198009
7. Nobiletin alleviates high-fat diet-induced nonalcoholic fatty liver disease by modulating AdipoR1 and gp91phox expression in rats.  |  Bunbupha, S., et al. 2021. J Nutr Biochem. 87: 108526. PMID: 33096235
8. Identification of neutrophil NADPH oxidase proteins gp91-phox, p22-phox, p67-phox, and p47-phox in mammalian species.  |  Hitt, ND. and Kleinberg, ME. 1996. Am J Vet Res. 57: 672-6. PMID: 8723880
9. Identification and characterization of TF1(phox), a DNA-binding protein that increases expression of gp91(phox) in PLB985 myeloid leukemia cells.  |  Eklund, EA. and Kakar, R. 1997. J Biol Chem. 272: 9344-55. PMID: 9083071
10. Biosynthesis of flavocytochrome b558. gp91(phox) is synthesized as a 65-kDa precursor (p65) in the endoplasmic reticulum.  |  Yu, L., et al. 1999. J Biol Chem. 274: 4364-9. PMID: 9933639

The preferred gp91phox/CYBB/NOX2 antibody is gp91phox/CYBB/NOX2 Antibody (54.1): sc-130543.