Anticorps gp91-phox (54.1) recommandé pour la détection de gp91-phox d'origine mouse, rat et human par WB, IP, IF et IHC(P)

Anticorps gp91-phox (54.1): sc-130543

Anticorps gp91-phox (54.1) est évalué 4.9 de 5 de 19.
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Fiches techniques

    • Anti-L'anticorps gp91-phox 54.1 est un monoclonal IgG1 (chaîne légère kappa) L'Anticorps gp91-phox fourni en 200 µg/ml
    • raised against gp91-phox of human origin, with epitope mapping to amino acids 383-390
    • Anti-L'Anticorps gp91-phox (54.1) est recommandé pour la détection de gp91-phox d'origine mouse, rat et human par WB, IP, IF et IHC(P); également recommandé pour la détection de ERp57, Mox1, Nox3 and Nox4
    • Anti-L'Anticorps gp91-phox (54.1) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • Voir m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nos recombinants vivement recommandés en alternative des réactifs IgG secondaires anti-souris conventionels.
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de gp91-phox (54.1): sc-130543.

Can I use this antibody conjugated with Phycoerythrin for IHC or IF? If yes, under which channel can I image the stained cells? Is there a protocol for IHC or IF for this antibody? Thank you

Posée par: Keertana
Thank you for your question. Anti-gp91-phox Antibody (54.1) and its conjugates are recommended for detection of gp91-phox of mouse, rat and human origin by WB, IP, IF and IHC(P). They are also recommended for detection of ERp57, Mox1, Nox3 and Nox4. You can find our recommended protocols for IHC and IF experiments under this link: https://www.scbt.com/resources/protocols PE absorption peaks in the visible light spectrum are measured at 495 and 545/566 nm.
Répondue par: Technical Support Europe
Date de publication: 2020-10-12

Is it possible for me to have a free trial sample for the above antibody? Thank you!

Posée par: Renato
Thank you very much for your inquiry. Please send us an email to [email protected] with your complete delivery address, we will contact you back as soon as possible.
Répondue par: Technical Support Europe
Date de publication: 2017-12-05

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Posée par: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-130543 PE) or fluoroscein (sc-130543 FITC), Alexa Fluor® 488 (sc-130543 AF488) or Alexa Fluor® 647 (sc-130543 AF647). Please contact Technical Service if you have further questions concerning this product.
Répondue par: Tech Service 9
Date de publication: 2017-01-04
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Rated 5 de 5 de par Very good for Western Blotting Works great for western blotting (mouse heart lysate), can see clear bands at about 60 kDa.
Date de publication: 2019-07-05
Rated 5 de 5 de par worked It works great - I 'd recommend this Nox2 antibody.
Date de publication: 2018-03-16
Rated 5 de 5 de par Great IB antibody This antibody works great with mouse cells. We also included Nox2-KO cells as a genetic control.
Date de publication: 2017-10-08
Rated 4 de 5 de par very good The strips are very clear and there are not many bands.
Date de publication: 2017-08-27
Rated 5 de 5 de par IF worked in murine DC 2.4 cells It worked great with murine DC 2.4 cells. I did not try to optimize the concentration, but it worked with 1:50 as they recommended.
Date de publication: 2017-07-08
Rated 5 de 5 de par This antibody work well for WB This antibody can also detect the samples from the pig ,it can replace the sc-5826 .
Date de publication: 2017-06-23
Rated 5 de 5 de par Awesome in WB Great signal in WB. Detects a single band in HeLa lysate at a 1:1000 dilution.
Date de publication: 2017-06-06
Rated 5 de 5 de par worked well in IHC Well working detect human breast tissue by IHC and IF, shows membrancing staining.
Date de publication: 2017-02-12
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