Date published: 2025-9-10

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Anticorps glypican-3 (1G12): sc-65443

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Fiches techniques
  • L'anticorps glypican-3 1G12 est un monoclonal IgG1 de souris, cité dans 30 publications, fourni en 15 µg/100 µl
  • élevé contre un fragment contenant les acides aminés 510-580 de glypican-3 d'origine human.
  • recommandé pour la détection de glypican-3 d'origine human par WB, IP, IF et IHC(P)
  • Voir glypican-3 (F-3): sc-390587 pour d'autres anticorps conjugués glypican-3, dont AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 et 790.
  • Nous testons actuellement encore nos anticorps secondaires pour trouver la meilleure protéine de liaison pour cet anticorps primaire glypican-3 (1G12). Veuillez nous contacter si vous avez des questions concernant les réactifs de détection secondaire appropriés.

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

Glypican-3 (GPC3) is an integral membrane protein that is mutated in the Simpson-Golabi-Behmel syndrome (SGBS). SGBS is characterized by pre- and post-natal overgrowth and is a recessive X-linked condition. Glypican-3, also designated OCI-5 in rat, is a member of the glypican family of heparan sulfate proteoglycans, which attach to the cell membrane via a glycosyl-phosphatidyl-inositol (GPI) anchor. Expression of glypican-3 is detected in embryonic mesodermal lung, liver and kidney tissues. Glypican-3 is thought to regulate tissue and organ growth through interactions with growth factors such as insulin-like growth factor II (IGF-II) or fibroblast growth factor 2 (FGF-2). Glypican-3 may be downregulated by various means, including promoter hypermethylation or the repression of specific transcription factors.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps glypican-3 (1G12)

sc-65443
15 µg/100 µl
$316.00

Não estou conseguindo detecção para WB, tem alguma recomendação de diluição mais especifica, de bloqueio e diluente para o anticorpo

Posée par: FFMF
Thank you for your question. Please contact our technical service team for troubleshooting advice on your assay.
Répondue par: Tech Support Europe
Date de publication: 2022-09-05

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Posée par: Sweetums
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Répondue par: Tech Service 4
Date de publication: 2016-12-16

I need to use an antibody to detect the protein in samples of (other species, e.g., bovine, porcine, canine, equine, avian, non-mammalian) origin. Do you know if this mouse monoclonal antibody will work?

Posée par: Sweetums
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Répondue par: Tech Service 4
Date de publication: 2016-12-16
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Rated 5 de 5 de par very strong signalNice western blot data, clear background effect is very good
Date de publication: 2017-05-23
Rated 5 de 5 de par Works great in Western Blot!This antibody works great in western blot analysis of glypican-3 expression in 293T whole cell lysate.
Date de publication: 2016-12-16
Rated 5 de 5 de par Works very well by IHC(P)Works very well by IHC(P) - Published data
Date de publication: 2015-08-04
Rated 5 de 5 de par Worked well in WB in human glypicanWorked well in WB in human glypican-3 transfected 293T whole cell lysate. -SCBT QC
Date de publication: 2015-01-07
Rated 5 de 5 de par ClearClear, strong band seen in WB in 293T whole cell lysate. -SCBT QC
Date de publication: 2013-06-16
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Anticorps glypican-3 (1G12) est évalué 5.0 de 5 de 5.
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