GILZ Antibody (D-2): sc-133216

GILZ Antibody (D-2) is a mouse monoclonal IgG1 antibody that detects glucocorticoid-induced leucine zipper (GILZ) in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GILZ plays a crucial role in mediating the immunosuppressive effects of glucocorticoid hormones, particularly in T cells, where GILZ expression is induced by dexamethasone. GILZ protects T cells from apoptosis induced by anti-CD3 antibodies by inhibiting the expression of Fas and Fas ligand, thereby promoting cell survival. GILZ′s ability to interact with key transcription factors, including Egr-2, Egr-3, and NFAT/AP-1, allows GILZ to modulate gene expression critical for T cell function. Notably, GILZ binds to c-Fos and c-Jun, which inhibits the binding of active AP-1 to its DNA consensus site, thus affecting downstream signaling pathways. Additionally, GILZ′s interaction with NFkB subunits prevents their nuclear translocation, further influencing immune responses. The gene encoding human GILZ is located on chromosome Xq22.3, and GILZ expression is predominantly found in normal lymphocytes from the thymus, spleen, and lymph nodes, while being absent in nonlymphoid tissues such as the brain, liver, and kidney. Anti-GILZ antibody (D-2) is an essential tool for researchers studying the complex regulatory mechanisms of immune responses and the role of glucocorticoids in T cell biology.

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GILZ Antibody (D-2) References:

1. Role of GILZ in immune regulation, glucocorticoid actions and rheumatoid arthritis.  |  Beaulieu, E. and Morand, EF. 2011. Nat Rev Rheumatol. 7: 340-8. PMID: 21556028
2. GILZ regulates Th17 responses and restrains IL-17-mediated skin inflammation.  |  Jones, SA., et al. 2015. J Autoimmun. 61: 73-80. PMID: 26077873
3. GILZ-dependent modulation of mTORC1 regulates spermatogonial maintenance.  |  La, HM., et al. 2018. Development. 145: PMID: 30126904
4. GILZ restrains neutrophil activation by inhibiting the MAPK pathway.  |  Ricci, E., et al. 2019. J Leukoc Biol. 105: 187-194. PMID: 30371949
5. Peripheral blood GILZ mRNA levels in depression and following electroconvulsive therapy.  |  Ryan, KM. and McLoughlin, DM. 2019. Psychoneuroendocrinology. 101: 304-310. PMID: 30602137
6. Role of GILZ in the Kidney and the Cardiovascular System: Relevance to Cardiorenal Complications of COVID-19.  |  Mozaffari, MS. 2020. J Pharmacol Exp Ther. 375: 398-405. PMID: 33008869
7. Exploiting the pro-resolving actions of glucocorticoid-induced proteins Annexin A1 and GILZ in infectious diseases.  |  Vago, JP., et al. 2021. Biomed Pharmacother. 133: 111033. PMID: 33378946
8. Expression Profiles of GILZ and Annexin A1 in Human Oral Candidiasis and Lichen Planus.  |  Mozaffari, MS. and Abdelsayed, R. 2022. Cells. 11: PMID: 35563776
9. GILZ regulates type I interferon release and sequesters STAT1.  |  Nataraja, C., et al. 2022. J Autoimmun. 131: 102858. PMID: 35810690
10. Association of GILZ with MUC2, TLR2, and TLR4 in Inflammatory Bowel Disease.  |  Cari, L., et al. 2023. Int J Mol Sci. 24: PMID: 36768553

The preferred GILZ antibody is GILZ Antibody (G-5): sc-133215.