Anticorps GDF-15 (H-2) recommandé pour la détection de precursor and mature GDF-15 d'origine mouse, rat et human par WB, IP, IF et ELISA

Anticorps GDF-15 (H-2): sc-515675

Anticorps GDF-15 (H-2) est évalué 4.0 de 5 de 1.
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Fiches techniques

    • L'Anticorps GDF-15 (H-2) est un monoclonal de souris IgG2a (chaîne légère kappa) fourni en 200 µg/ml
    • spécifique d'un épitope situé entre les acides aminés 41-62 près de N-terminus de d'origine rat
    • Anti-L'Anticorps GDF-15 (H-2) est recommandé pour la détection de precursor and mature GDF-15 d'origine mouse, rat et human par WB, IP, IF et ELISA
    • Anti-L'Anticorps GDF-15 (H-2) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de GDF-15 (H-2): sc-515675.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for GDF-15 Antibody (H-2) for WB applications. This reagent is now offered in a bundle with GDF-15 Antibody (H-2) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Please tell me the blocking peptide corresponding to this antibody (sc-515675).

Posée par: yuki13
Thank you for your question. We do not offer a blocking peptide for this antibody.
Répondue par: Tech Support Europe
Date de publication: 2020-07-29

I have a question. is western blot data non-reducing conditions or reducing conditions? I did WB to detect gdf15 in LNCaP by non-reducing conditions. I got 2 band : one above 40 kDa, the other around 35 kDa.

Posée par: Col12
Thank you for your question. Our Western blot data with this antibody is under reducing conditions. We have not tested the antibody under non-reducing conditions.
Répondue par: Technical Support
Date de publication: 2019-05-25

I have a rat cell line, can this ab detect GDF15 from rat?

Posée par: M76gmm
Thank you for your question. Yes, this antibody is recommended for detection of GDF15 of mouse, rat and human origin. We have Western blot data on our website in the rat cell line, RIN-m5F.
Répondue par: Technical Support
Date de publication: 2019-01-31

Has anyone any information that this Ab can detect human GDF15. According to the protein alignments in aa 41-62 the Human sequence differs by an additional 7 amino acids. Would be keen to see some experimental data if anyone has it. Thanks

Posée par: Satish Patel
Thank you for your question. Yes, we have human cell data on this antibody in LNCaP cells on the product page: https://www.scbt.com/scbt/product/gdf-15-antibody-h-2?requestFrom=search If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Répondue par: Tech Service
Date de publication: 2017-06-28

I need to use an antibody to detect the protein in samples of avian origin. Do you know if sc-515675: GDF-15 (H-2) mouse monoclonal antibody will work?

Posée par: Dr Ninau Qelp
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Répondue par: Technical Support
Date de publication: 2017-03-06
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Rated 4 de 5 de par Works well on murine cardiac samples 1h blocking in mPBST, GDF15 ab ON at 4°C, secondary ab 1 h at RT; Ab detected 2 bands: one slightly above 40 kDa, the other around 25 kDa
Date de publication: 2017-11-27
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