GADD 45α Antibody (C-4): sc-6850

GADD 45α Antibody (C-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects GADD 45 alpha in mouse, rat, and human samples through various applications including western blotting, immunoprecipitation, immunofluorescence, flow cytometry (FCM), and enzyme-linked immunosorbent assay. GADD 45α (C-4) antibody is offered in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and various Alexa Fluor® conjugates. GADD 45 alpha plays a crucial role in the cellular response to DNA damage, particularly in regulating cell cycle checkpoints. GADD 45 alpha interacts with cyclin-dependent kinases (Cdks) and proliferating cell nuclear antigen (PCNA), which are essential for DNA replication and repair processes. GADD 45 alpha helps to inhibit Cdk activity by binding to these proteins, thereby preventing cell cycle progression into the S phase when DNA damage is present. This function allows cells to repair DNA before division, thus maintaining genomic stability and preventing the propagation of damaged DNA, which can lead to tumorigenesis. GADD 45 alpha stimulates DNA excision repair, further underscoring its importance in cellular defense mechanisms against genotoxic stress.

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GADD 45α Antibody (C-4) References:

1. Wild-type p53 is a cell cycle checkpoint determinant following irradiation.  |  Kuerbitz, SJ., et al. 1992. Proc Natl Acad Sci U S A. 89: 7491-5. PMID: 1323840
2. Creative blocks: cell-cycle checkpoints and feedback controls.  |  Murray, AW. 1992. Nature. 359: 599-604. PMID: 1406993
3. A mammalian cell cycle checkpoint pathway utilizing p53 and GADD45 is defective in ataxia-telangiectasia.  |  Kastan, MB., et al. 1992. Cell. 71: 587-97. PMID: 1423616
4. NF-kappa B-mediated repression of growth arrest- and DNA-damage-inducible proteins 45alpha and gamma is essential for cancer cell survival.  |  Zerbini, LF., et al. 2004. Proc Natl Acad Sci U S A. 101: 13618-23. PMID: 15353598
5. Diverse roles of GADD45alpha in stress signaling.  |  Gao, M., et al. 2009. Curr Protein Pept Sci. 10: 388-94. PMID: 19689359
6. Regulatory Roles of GADD45α in Skeletal Muscle and Adipocyte.  |  You, W., et al. 2019. Curr Protein Pept Sci. 20: 918-925. PMID: 31232235
7. Harmine inhibits pulmonary fibrosis through regulating DNA damage repair-related genes and activation of TP53-Gadd45α pathway.  |  Gong, Y., et al. 2024. Int Immunopharmacol. 138: 112542. PMID: 38924867
8. New link found between p53 and DNA repair.  |  Marx, J. 1994. Science. 266: 1321-2. PMID: 7973719
9. Interaction of the p53-regulated protein Gadd45 with proliferating cell nuclear antigen.  |  Smith, ML., et al. 1994. Science. 266: 1376-80. PMID: 7973727
10. Induction of WAF1/CIP1 by a p53-independent pathway.  |  Michieli, P., et al. 1994. Cancer Res. 54: 3391-5. PMID: 8012956
11. WAF1/CIP1 is induced in p53-mediated G1 arrest and apoptosis.  |  el-Deiry, WS., et al. 1994. Cancer Res. 54: 1169-74. PMID: 8118801
12. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases.  |  Harper, JW., et al. 1993. Cell. 75: 805-16. PMID: 8242751

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GADD 45α Antibody (C-4) has 48 citations in a variety of scientific publications.

Primary antibodies like Anti-GADD 45α Antibody (C-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GADD 45α Antibody (C-4) Specifications

Alternate Product Name: GADD 45 alpha

Product Clone Name: C-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-165

Epitope Target: GADD 45α

Epitope Species: human

Product Reactivity/Detection: GADD 45α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 48 citations in various scientific publications

Available Conjugates/Forms for GADD 45α Antibody (C-4): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin