Anticorps CyPA (6-YD13) recommandé pour la détection de CyPA d'origine mouse, rat et human par WB, IP et ELISA

Anticorps CyPA (6-YD13): sc-134310

Anticorps CyPA (6-YD13) est évalué 4.2 de 5 de 5.
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Fiches techniques

    • Anti-L'anticorps CyPA 6-YD13 est un monoclonal IgG2a κ L'Anticorps CyPA, cité dans 9 publications, fourni en 100 µg/ml
    • dirigé contre la protéine totale CyPA de human
    • recommandé pour la détection de CyPA d'origine mouse, rat et human par WB, IP et ELISA
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de CyPA (6-YD13): sc-134310.
    • m-IgG Fc BP-HRP et m-IgG2a BP-HRP sont les réactifs secondaires recommandés pour la détection de l'anticorps CyPA (6-YD13) pour des applications de WB. Ces réactifs sont maintenant proposés en kit avec l'anticorps CyPA (6-YD13) (voir Informations pour la commande ci-dessous).
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What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Posée par: jerojero
Thank you for your question We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Répondue par: Santa Cruz TS
Date de publication: 2017-01-27
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Rated 1 de 5 de par Not nearly as good as their old rabbit polyclonal! I did an IP to pull down CypA (using abcam's antibody as its better), and then came back with this 6-YD13 mouse monoclonal antibody (right hand blot on image). This is in HEK cells. The antibody band for CypA is very weak signal and the antibody bands for the light chain and heavy chain from the IP beads is very overpowering- I never saw this with the old CypA antibody. I thought that maybe the IP extract is degraded (unlikely though as I've done this lots of times before using my protocol). So I reprobed the same blot with the old C-14 polyclonal rabbit antibody I had left over and the bands for CypA are clear as day . Both antibodies were used at 1:1000 diluted in 5% BSA PBST. I can't increase the amount of 6-YD13 used as it will also increase the signal of the light/heavy chain and will bleach the CypA signal more.
Date de publication: 2017-07-17
Rated 5 de 5 de par Strong signal in WB Very clear band in WB at 1:500 dilution. Several publications available for WB and IF in mouse and human samples.
Date de publication: 2016-12-21
Rated 5 de 5 de par powerful tool Made outstanding contribution in my work and paper。
Date de publication: 2016-11-03
Rated 5 de 5 de par Robust band in western blot analysis of CyPA Robust band in western blot analysis of CyPA expression in K-562 whole cell lysate. -SCBT QC
Date de publication: 2015-03-11
Rated 5 de 5 de par Strong band present in western blot using Strong band present in western blot using Jurkat whole cell lysate. -SCBT QC
Date de publication: 2013-10-04
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