CUL-3 Antibody (G-8): sc-166110

CUL-3 Antibody (G-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects CUL-3 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). CUL-3 (G-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CUL-3 plays a crucial role in the ubiquitin-proteasome system, specifically in the selective degradation of target proteins, which is vital for maintaining cellular homeostasis and regulating various biological processes, including cell cycle progression and signal transduction. CUL-3 functions as a component of the E3 ubiquitin ligase complex, facilitating the ubiquitination of specific substrates, thereby influencing their stability and activity. Notably, CUL-3 is involved in the degradation of cyclin E, a key regulator of the cell cycle, independent of the SCF complex, highlighting a unique role in cellular regulation. CUL-3′s ability to modulate protein levels is essential for processes such as differentiation, development, and response to stress, making CUL-3 a significant target for research in cancer and other diseases where protein homeostasis is disrupted.

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CUL-3 Antibody (G-8) References:

1. One ring to rule a superfamily of E3 ubiquitin ligases.  |  Tyers, M. and Willems, AR. 1999. Science. 284: 601, 603-4. PMID: 10328744
2. Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells.  |  Singer, JD., et al. 1999. Genes Dev. 13: 2375-87. PMID: 10500095
3. Identification of the von Hippel-lindau tumor-suppressor protein as part of an active E3 ubiquitin ligase complex.  |  Iwai, K., et al. 1999. Proc Natl Acad Sci U S A. 96: 12436-41. PMID: 10535940
4. Identification and characterization of cul-3b, a novel hominine CUL-3 transcript variant.  |  Lu, L., et al. 2005. Asian J Androl. 7: 205-11. PMID: 15897978
5. The BTB protein MEL-26 promotes cytokinesis in C. elegans by a CUL-3-independent mechanism.  |  Luke-Glaser, S., et al. 2005. Curr Biol. 15: 1605-15. PMID: 16169482
6. Prospect of divergent roles for the CUL3 system in vascular endothelial cell function and angiogenesis.  |  Sakaue, T., et al. 2017. J Biochem. 162: 237-245. PMID: 28981750
7. Structural and biochemical characterization establishes a detailed understanding of KEAP1-CUL3 complex assembly.  |  Adamson, RJ., et al. 2023. Free Radic Biol Med. 204: 215-225. PMID: 37156295
8. CUL3 induces mitochondrial dysfunction via MRPL12 ubiquitination in renal tubular epithelial cells.  |  Ji, X., et al. 2023. FEBS J. 290: 5340-5352. PMID: 37526061
9. cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family.  |  Kipreos, ET., et al. 1996. Cell. 85: 829-39. PMID: 8681378
10. Identification and analysis of expression of human VACM-1, a cullin gene family member located on chromosome 11q22-23.  |  Byrd, PJ., et al. 1997. Genome Res. 7: 71-5. PMID: 9037604
11. The human homologue for the Caenorhabditis elegans cul-4 gene is amplified and overexpressed in primary breast cancers.  |  Chen, LC., et al. 1998. Cancer Res. 58: 3677-83. PMID: 9721878
12. Human CUL-1 associates with the SKP1/SKP2 complex and regulates p21(CIP1/WAF1) and cyclin D proteins.  |  Yu, ZK., et al. 1998. Proc Natl Acad Sci U S A. 95: 11324-9. PMID: 9736735

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CUL-3 Antibody (G-8) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-CUL-3 Antibody (G-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CUL-3 Antibody (G-8) Specifications

Alternate Product Name: CUL-3

Product Clone Name: G-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-293

Epitope Target: CUL-3 (cullin-3)

Epitope Species: human

Product Reactivity/Detection: CUL-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for CUL-3 Antibody (G-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin