PFK-2/PFKFB2 Antibody (D-1): sc-377416

PFK-2 car Antibody (D-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects PFK-2 car in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PFK-2 car Antibody (D-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PFK-2 car (D-1) antibody targets a protein that plays a crucial role in glycolysis regulation by synthesizing fructose-2,6-bisphosphate, a potent allosteric activator of phosphofructokinase-1 (PFK-1). This regulation is vital because PFK-1 is a key control point in the glycolytic pathway, influencing the conversion of fructose 6-phosphate and ATP into fructose 1,6-bisphosphate, thereby impacting cellular energy production and metabolism. PFK-2 car monoclonal antibody (D-1) recognizes a bifunctional enzyme that not only synthesizes fructose-2,6-bisphosphate but also possesses fructose-2,6-bisphosphatase activity, allowing for degradation of this metabolite when necessary, thus providing a fine-tuned balance in metabolic control. The dimeric structure of PFK-2 and its various isoenzymes, including PFK-2 liver (PFKFB1), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3), and PFK-2 testis (PFKFB4), highlight its diverse regulatory roles across different tissues, making anti-PFK-2 car antibody (D-1) an essential tool for studying metabolic pathways and their implications in health and disease.

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PFK-2/PFKFB2 Antibody (D-1) References:

1. PFK-2/FBPase-2: maker and breaker of the essential biofactor fructose-2,6-bisphosphate.  |  Okar, DA., et al. 2001. Trends Biochem Sci. 26: 30-5. PMID: 11165514
2. Role of Ser530, Arg292, and His662 in the allosteric behavior of rabbit muscle phosphofructokinase.  |  Chang, SH. and Kemp, RG. 2002. Biochem Biophys Res Commun. 290: 670-5. PMID: 11785951
3. Changes in activity and expression of phosphofructokinase in different rat brain regions after basal forebrain cholinergic lesion.  |  Zeitschel, U., et al. 2002. J Neurochem. 83: 371-80. PMID: 12423247
4. The a-subunit of the V-type H+-ATPase interacts with phosphofructokinase-1 in humans.  |  Su, Y., et al. 2003. J Biol Chem. 278: 20013-8. PMID: 12649290
5. Phosphofructokinase muscle-specific isoform requires caveolin-3 expression for plasma membrane recruitment and caveolar targeting: implications for the pathogenesis of caveolin-related muscle diseases.  |  Sotgia, F., et al. 2003. Am J Pathol. 163: 2619-34. PMID: 14633633
6. No spontaneous second wind in muscle phosphofructokinase deficiency.  |  Haller, RG. and Vissing, J. 2004. Neurology. 62: 82-6. PMID: 14718702
7. Novel testis- and embryo-specific isoforms of the phosphofructokinase-1 muscle type gene.  |  Yamada, S., et al. 2004. Biochem Biophys Res Commun. 316: 580-7. PMID: 15020257
8. Interaction of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase-2) with glucokinase activates glucose phosphorylation and glucose metabolism in insulin-producing cells.  |  Massa, L., et al. 2004. Diabetes. 53: 1020-9. PMID: 15047617
9. Interaction of calmodulin with the phosphofructokinase target sequence.  |  Martin, SR., et al. 2004. FEBS Lett. 577: 284-8. PMID: 15527800
10. Expression of caveolin-1 in lymphocytes induces caveolae formation and recruitment of phosphofructokinase to the plasma membrane.  |  Vallejo, J. and Hardin, CD. 2005. FASEB J. 19: 586-7. PMID: 15665033
11. Covalent control of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase: insights into autoregulation of a bifunctional enzyme.  |  Kurland, IJ. and Pilkis, SJ. 1995. Protein Sci. 4: 1023-37. PMID: 7549867
12. Endogenous nitric oxide inhibits glucose-induced insulin secretion by suppression of phosphofructokinase activity in pancreatic islets.  |  Tsuura, Y., et al. 1998. Biochem Biophys Res Commun. 252: 34-8. PMID: 9813142

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PFK-2/PFKFB2 Antibody (D-1) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-PFK-2/PFKFB2 Antibody (D-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PFK-2/PFKFB2 Antibody (D-1) Specifications

Alternate Product Name: PFKFB2

Product Clone Name: D-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 75-105

Epitope Target: PFKFB2

Epitope Species: human

Product Reactivity/Detection: PFKFB2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for PFK-2/PFKFB2 Antibody (D-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin