HIV-1 Integrase Antibody (IN-2): sc-69721
- HIV-1 Integrase Antibody (IN-2) is a mouse monoclonal IgG1 κ HIV-1 Integrase antibody, cited in 11 publications, provided at 200 µg/ml
- raised against HIV-1 Integrase
- HIV-1 Integrase Antibody (IN-2) is recommended for detection of Pol Integrase of HIV-1 origin by WB, IP and IF
- Anti-HIV-1 Integrase Antibody (IN-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for HIV-1 Integrase Antibody (IN-2) for WB applications. These reagents are now offered in bundles with HIV-1 Integrase Antibody (IN-2) (see ordering information below).
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HIV-1 Integrase Antibody (IN-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects HIV-1 Integrase protein of HIV-1 origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-HIV-1 Integrase antibody (IN-2) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HIV-1 Integrase plays a crucial role in viral life cycle by facilitating viral DNA integration into host cell′s genome, a process essential for virus replication. This integration enables virus evasion of host immune response by becoming part of host genetic material. HIV-1 Integrase operates through precise DNA cleaving and ligation reactions, critical for accurate viral DNA insertion into host genome. HIV-1 Integrase (IN-2) antibody recognizes a protein whose structural integrity is maintained by C-terminal domain, forming a homodimer resembling Src-homology 3 (SH3) domains, while N-terminal domain contains conserved HHCC motif essential for zinc binding and protein stability. This unique structural configuration underscores HIV-1 Integrase importance in viral replication and presents potential therapeutic targets for HIV treatment strategies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HIV-1 Integrase Antibody (IN-2) References:
- HIV-1 infection requires a functional integrase NLS. | Bouyac-Bertoia, M., et al. 2001. Mol Cell. 7: 1025-35. PMID: 11389849
- The DNA-binding domain of HIV-1 integrase has an SH3-like fold. | Eijkelenboom, AP., et al. 1995. Nat Struct Biol. 2: 807-10. PMID: 7552753
- Crystal structure of the catalytic domain of HIV-1 integrase: similarity to other polynucleotidyl transferases. | Dyda, F., et al. 1994. Science. 266: 1981-6. PMID: 7801124
- Binding and stimulation of HIV-1 integrase by a human homolog of yeast transcription factor SNF5. | Kalpana, GV., et al. 1994. Science. 266: 2002-6. PMID: 7801128
- Identification of discrete functional domains of HIV-1 integrase and their organization within an active multimeric complex. | Engelman, A., et al. 1993. EMBO J. 12: 3269-75. PMID: 8344264
- Zinc folds the N-terminal domain of HIV-1 integrase, promotes multimerization, and enhances catalytic activity. | Zheng, R., et al. 1996. Proc Natl Acad Sci U S A. 93: 13659-64. PMID: 8942990
- Solution structure of the N-terminal zinc binding domain of HIV-1 integrase. | Cai, M., et al. 1997. Nat Struct Biol. 4: 567-77. PMID: 9228950
- HIV-1 infection of nondividing cells through the recognition of integrase by the importin/karyopherin pathway. | Gallay, P., et al. 1997. Proc Natl Acad Sci U S A. 94: 9825-30. PMID: 9275210
- Three new structures of the core domain of HIV-1 integrase: an active site that binds magnesium. | Goldgur, Y., et al. 1998. Proc Natl Acad Sci U S A. 95: 9150-4. PMID: 9689049
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HIV-1 Integrase Antibody (IN-2) | sc-69721 | 200 µg/ml | $322.00 | |||
HIV-1 Integrase Antibody (IN-2): m-IgG Fc BP-HRP Bundle | sc-526982 | 200 µg Ab; 10 µg BP | $361.00 | |||
HIV-1 Integrase Antibody (IN-2): m-IgG1 BP-HRP Bundle | sc-532355 | 200 µg Ab; 20 µg BP | $361.00 | |||
HIV-1 Integrase Antibody (IN-2) AC | sc-69721 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HIV-1 Integrase Antibody (IN-2) HRP | sc-69721 HRP | 200 µg/ml | $322.00 | |||
HIV-1 Integrase Antibody (IN-2) FITC | sc-69721 FITC | 200 µg/ml | $336.00 | |||
HIV-1 Integrase Antibody (IN-2) PE | sc-69721 PE | 200 µg/ml | $349.00 | |||
HIV-1 Integrase Antibody (IN-2) Alexa Fluor® 488 | sc-69721 AF488 | 200 µg/ml | $364.00 | |||
HIV-1 Integrase Antibody (IN-2) Alexa Fluor® 546 | sc-69721 AF546 | 200 µg/ml | $364.00 | |||
HIV-1 Integrase Antibody (IN-2) Alexa Fluor® 594 | sc-69721 AF594 | 200 µg/ml | $364.00 | |||
HIV-1 Integrase Antibody (IN-2) Alexa Fluor® 647 | sc-69721 AF647 | 200 µg/ml | $364.00 | |||
HIV-1 Integrase Antibody (IN-2) Alexa Fluor® 680 | sc-69721 AF680 | 200 µg/ml | $364.00 | |||
HIV-1 Integrase Antibody (IN-2) Alexa Fluor® 790 | sc-69721 AF790 | 200 µg/ml | $364.00 |