HDAC1 Anticuerpo (10E2) recomendado para detectarHDAC1 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA

HDAC1 Anticuerpo (10E2): sc-81598

HDAC1 Anticuerpo (10E2) clasificado 4.9 de 5 por 33.
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Ficha técnica

    • Anti-HDAC1 Anticuerpo 10E2 es un monoclonal de ratón IgG1 κ HDAC1 Anticuerpo, ver las 92 publicaciones, proporcionado como 200 µg/ml
    • producido contra los amino ácidos 467-482a de HDAC1 de origen human
    • HDAC1 Anticuerpo (10E2) es recomendado para detectar HDAC1 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA; no tiene reactividad cruzada con other HDAC proteins
    • HDAC1 Anticuerpo (10E2) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • TransCruz reactivo para Gel Supershift y ChIP (sc-81598 X, 200 µg/0.1 ml)
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de HDAC1 (10E2): sc-81598.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for HDAC1 Antibody (10E2) for WB and IHC(P) applications. This reagent is now offered in a bundle with HDAC1 Antibody (10E2) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

What is the recommended buffer for tis antibody? Is it PBST based or TBST based? Thank you.

Preguntado por: bam289
Thank you for your question. For most applications, we recommend using a TBST based buffer with this antibody. A link to a complete list of our recommended protocols is provided below as a reference. https://www.scbt.com/scbt/resources/protocols
Respondida por: Technical Support
Fecha de publicación: 2017-10-24

What is the recommended fixation method for IF with HDAC1 Antibody (10E2): sc-81598?

Preguntado por: jenni
We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Respondida por: Technical Support 12
Fecha de publicación: 2017-01-09
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Rated 5 de 5 por de Really good antibody Really good antibody for WB and IF applications. Used with nuclear extract and whole cell extract from human cell lines.
Fecha de publicación: 2020-08-04
Rated 5 de 5 por de HDAC1 SINGLE BAND DETECTED WITH THE EXPECTED MOLECULAR MASS WITH AB DILUTION OF 1:5000
Fecha de publicación: 2019-09-12
Rated 5 de 5 por de Good response in WB This product shows good reactivity with WB using HEK 293 lysate.
Fecha de publicación: 2019-03-14
Rated 5 de 5 por de Dr Sadek Ab work good with dilution 1:200 and 2 hrs incubation at 37ºc and overnight at 4 ºc
Fecha de publicación: 2018-11-21
Rated 5 de 5 por de Highly-recommended antibody for HDAC1 I use this antibody to detect 3D pancreatic cancer cell line and cancer-related fibroblasts in WB and the results are very clear and clean. It could be used even at 1:5000 as attached! The green bands in the attached are detected by HDAC1 antibody (10E2) and the red ones are detected by SMA from another company.
Fecha de publicación: 2018-04-27
Rated 4 de 5 por de HDAC1, works ok We used this antibody for chemiluminescence Western Blot on mouse embryo nuclear lysate, 1:600 worked well, but a lower concentration would work better.
Fecha de publicación: 2018-04-23
Rated 5 de 5 por de works well for western worked well for western using Urea lysis buffer. We would order again.
Fecha de publicación: 2018-02-26
Rated 5 de 5 por de Good results I try this antibody sample free. It works vero well in WB performed using rat liver nuclear lysate
Fecha de publicación: 2017-10-13
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