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GTBP Antibody (E-8) - Western Blotting - Image 164447
GTBP Antibody (E-8): sc-137015. Western blot analysis of GTBP expression in HeLa (A), A-431 (B), SW480 (C), K-562 (D), DU 145 (E) and Jurkat (F) nuclear extracts.
GTBP Antibody (E-8): sc-137015. Western blot analysis of GTBP expression in HeLa (A), A-431 (B), SW480 (C), K-562 (D), DU 145 (E) and Jurkat (F) nuclear extracts.

GTBP Antibody (E-8): sc-137015

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Datasheets
  • GTBP Antibody (E-8) is a mouse monoclonal IgG2a κ GTBP antibody, cited in 3 publications, provided at 200 µg/ml
  • raised against amino acids 1220-1360 mapping at the C-terminus of GTBP (G/T binding protein) of human origin
  • GTBP Antibody (E-8) is recommended for detection of GTBP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • Anti-GTBP Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GTBP Antibody (E-8) for WB and IHC(P) applications. These reagents are now offered in bundles with GTBP Antibody (E-8) (see ordering information below).
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SEE ALSO...

GTBP Antibody (E-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects GTBP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). GTBP (E-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. GTBP, also known as MSH6, plays a crucial role in DNA mismatch repair pathway, which maintains genomic stability by correcting DNA replication errors. GTBP is primarily located in the nucleus, where GTBP forms a heterodimer with MSH2 to recognize and bind to G/T mismatches in DNA. This interaction initiates a cascade of events leading to excision and resynthesis of erroneous DNA segments. GTBP′s importance in this pathway is underscored by GTBP′s association with hereditary nonpolyposis colorectal cancer (HNPCC), where mutations in mismatch repair genes lead to microsatellite instability and increased cancer risk. Using anti-GTBP antibody (E-8) in research can provide valuable insights into DNA repair mechanisms and their dysfunction in cancer biology.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

GTBP Antibody (E-8) References:

  1. hMSH2 and GTBP expression in advanced stage epithelial ovarian cancer.  |  Ercoli, A., et al. 1999. Br J Cancer. 80: 1665-71. PMID: 10408416
  2. Mismatch repair genes (hMLH1, hPMS1, hPMS2, GTBP/hMSH6, hMSH2) in the pathogenesis of hepatocellular carcinoma.  |  Zekri, AR., et al. 2005. World J Gastroenterol. 11: 3020-6. PMID: 15918183
  3. GTBP, a 160-kilodalton protein essential for mismatch-binding activity in human cells.  |  Palombo, F., et al. 1995. Science. 268: 1912-4. PMID: 7604265
  4. Mutations of GTBP in genetically unstable cells.  |  Papadopoulos, N., et al. 1995. Science. 268: 1915-7. PMID: 7604266
  5. MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast.  |  Prolla, TA., et al. 1994. Science. 265: 1091-3. PMID: 8066446
  6. Mutations of two PMS homologues in hereditary nonpolyposis colon cancer.  |  Nicolaides, NC., et al. 1994. Nature. 371: 75-80. PMID: 8072530
  7. Mismatch repair and cancer.  |  Palombo, F., et al. 1994. Nature. 367: 417. PMID: 8107798
  8. Mutation of a mutL homolog in hereditary colon cancer.  |  Papadopoulos, N., et al. 1994. Science. 263: 1625-9. PMID: 8128251
  9. Mutation in the DNA mismatch repair gene homologue hMLH1 is associated with hereditary non-polyposis colon cancer.  |  Bronner, CE., et al. 1994. Nature. 368: 258-61. PMID: 8145827
  10. Genetic mapping of a locus predisposing to human colorectal cancer.  |  Peltomäki, P., et al. 1993. Science. 260: 810-2. PMID: 8484120
  11. Germ-line mutation of the hMSH6/GTBP gene in an atypical hereditary nonpolyposis colorectal cancer kindred.  |  Akiyama, Y., et al. 1997. Cancer Res. 57: 3920-3. PMID: 9307272
  12. Alternative splicing of GTBP in normal human tissues.  |  Shiwaku, HO., et al. 1997. DNA Res. 4: 359-62. PMID: 9455487

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

GTBP Antibody (E-8)

sc-137015
200 µg/ml
$322.00

GTBP Antibody (E-8): m-IgG Fc BP-HRP Bundle

sc-528837
200 µg Ab; 10 µg BP
$361.00

GTBP Antibody (E-8): m-IgGκ BP-HRP Bundle

sc-521395
200 µg Ab, 40 µg BP
$361.00

GTBP Antibody (E-8): m-IgG2a BP-HRP Bundle

sc-547181
200 µg Ab; 10 µg BP
$361.00

GTBP Antibody (E-8) AC

sc-137015 AC
500 µg/ml, 25% agarose
$424.00

GTBP Antibody (E-8) HRP

sc-137015 HRP
200 µg/ml
$322.00

GTBP Antibody (E-8) FITC

sc-137015 FITC
200 µg/ml
$336.00

GTBP Antibody (E-8) PE

sc-137015 PE
200 µg/ml
$349.00

GTBP Antibody (E-8) Alexa Fluor® 488

sc-137015 AF488
200 µg/ml
$364.00

GTBP Antibody (E-8) Alexa Fluor® 546

sc-137015 AF546
200 µg/ml
$364.00

GTBP Antibody (E-8) Alexa Fluor® 594

sc-137015 AF594
200 µg/ml
$364.00

GTBP Antibody (E-8) Alexa Fluor® 647

sc-137015 AF647
200 µg/ml
$364.00

GTBP Antibody (E-8) Alexa Fluor® 680

sc-137015 AF680
200 µg/ml
$364.00

GTBP Antibody (E-8) Alexa Fluor® 790

sc-137015 AF790
200 µg/ml
$364.00

Can GTBP (E-8): sc-137015 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jerojero
Thank you for your question. Yes, GTBP (E-8): sc-137015 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Great featureI requested this antibody for free a couple weeks ago, the first time I run WB as 1:100, it works well, but has some non-specific bands. After that, the antibody works fine, one specific band appears around 180kD in my Hela cell samples in shorter exposure time.
Date published: 2017-10-03
Rated 3 out of 5 by from GTBP/Msh6 antibody (E-8) in Western blottingI received a sample antibody and tried it at a 1:100 dilution to probe whole cell extracts from various cell lines as labelled in my picture (note that GTBP runs at ~160 kDa). While it did not work in Mouse Embryonic Fibroblast (MEF) cells, it did work in human cell lines like HeLa, HEK293, and HEK293T (also known as Phoenix cells). There were some non-specific bands from this antibody, as seen in my western blot. If I were to buy this antibody or receive another sample, I might try a lower concentration next time. Overall, I'd say it's not bad.
Date published: 2017-09-05
Rated 5 out of 5 by from GreatThe antibody is pretty good for IF staining. The background is very low
Date published: 2017-08-02
Rated 5 out of 5 by from Produced excellent immunoperoxidase nuclearProduced excellent immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human lymph node tissue. -SCBT QC
Date published: 2015-02-02
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GTBP Antibody (E-8) is rated 4.6 out of 5 by 5.
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