GRASP55 Antibody (E-11): sc-365602
- GRASP55 Antibody (E-11) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 429-449 at the C-terminus of GRASP55 of rat origin
- recommended for detection of GRASP55 of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for GRASP55 Antibody (E-11) for WB applications. This reagent is now offered in a bundle with GRASP55 Antibody (E-11) (see ordering information below).
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GRASP55 Antibody (E-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects GRASP55 protein of mouse, rat, and human by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GRASP55, also known as Golgi reassembly stacking protein or p59, plays a crucial role in the organization and maintenance of the Golgi apparatus, a vital organelle responsible for processing and packaging proteins for secretion. The Golgi apparatus is structured as a series of stacked cisternae, and GRASP55 is integral to the stacking machinery, working alongside GRASP65. GRASP55 undergoes myristoylation and palmitoylation, which are important post-translational modifications that influence membrane association and function. Unlike GRASP65, GRASP55 does not bind to the vesicle docking protein GM130 and primarily localizes to the medial-Golgi, highlighting a specific role in Golgi architecture. GRASP55 interacts with golgin 45 and the GTP-bound form of Rab 2, forming a complex essential for normal protein transport and Golgi structure. ERK2 phosphorylation of GRASP55 during mitosis indicates involvement in the regulation of Golgi dynamics in response to cellular signaling, underscoring importance in maintaining cellular homeostasis and function.
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GRASP55 Antibody (E-11) References:
- GRASP55 regulates Golgi ribbon formation. | Feinstein, TN. and Linstedt, AD. 2008. Mol Biol Cell. 19: 2696-707. PMID: 18434598
- GRASP55 Senses Glucose Deprivation through O-GlcNAcylation to Promote Autophagosome-Lysosome Fusion. | Zhang, X., et al. 2018. Dev Cell. 45: 245-261.e6. PMID: 29689198
- GRASP55 facilitates autophagosome maturation under glucose deprivation. | Zhang, X. and Wang, Y. 2018. Mol Cell Oncol. 5: e1494948. PMID: 30250930
- Unconventional secretion factor GRASP55 is increased by pharmacological unfolded protein response inducers in neurons. | van Ziel, AM., et al. 2019. Sci Rep. 9: 1567. PMID: 30733486
- GRASP55 and UPR Control Interleukin-1β Aggregation and Secretion. | Chiritoiu, M., et al. 2019. Dev Cell. 49: 145-155.e4. PMID: 30880003
- SIRT2 deacetylates GRASP55 to facilitate post-mitotic Golgi assembly. | Zhang, X., et al. 2019. J Cell Sci. 132: PMID: 31604796
- Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the Golgi structure. | Zhang, Y. and Seemann, J. 2021. J Cell Biol. 220: PMID: 33301566
- The Golgi stacking protein GRASP55 is targeted by the natural compound prodigiosin. | Berning, L., et al. 2023. Cell Commun Signal. 21: 275. PMID: 37798768
- Myristoylated GRASP55 dimerizes in the presence of model membranes. | Kava, E., et al. 2024. J Biomol Struct Dyn. 1-12. PMID: 38361284
- GRASP55 Regulates Sorting and Maturation of the Lysosomal Enzyme β-Hexosaminidase A. | Akaaboune, SR., et al. 2024. bioRxiv.. PMID: 39464054
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GRASP55 Antibody (E-11) | sc-365602 | 200 µg/ml | $322.00 | |||
GRASP55 Antibody (E-11): m-IgG Fc BP-HRP Bundle | sc-552842 | 200 µg Ab; 10 µg BP | $361.00 | |||
GRASP55 (E-11) Neutralizing Peptide | sc-365602 P | 100 µg/0.5 ml | $69.00 |