ASL Anticuerpo (B-1) recomendado para detectarASL de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA

ASL Anticuerpo (B-1): sc-166787

ASL Anticuerpo (B-1) clasificado 4.3 de 5 por 4.
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Ficha técnica

    • Anti-ASL Anticuerpo B-1 es un monoclonal de ratón IgG1 κ ASL Anticuerpo, ver las 1 publicaciones, proporcionado como 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of ASL of human origin
    • ASL Anticuerpo (B-1) es recomendado para detectar ASL de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA
    • ASL Anticuerpo (B-1) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de ASL (B-1): sc-166787.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ASL Antibody (B-1) for WB and IHC(P) applications. These reagents are now offered in bundles with ASL Antibody (B-1) (see ordering information below).
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I am using ASL (B-1): sc-166787 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Preguntado por: jenniferc
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Respondida por: Technical Support
Fecha de publicación: 2017-02-24
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Rated 2 de 5 por de Poor performance I have recently purchased this antibody and it's not shown a very good performance. I have used it for fluorescence WB where it showed a faint band for ASL and a lot of unspecific background. I have tested the antibody for IF in fixed Hap1 cells, but the fluorescence signal was incredibly low. Not impressed at all.
Fecha de publicación: 2020-09-29
Rated 5 de 5 por de Good for WB This antibody showed nice signal from hepatocytes of mice and human
Fecha de publicación: 2017-01-27
Rated 5 de 5 por de WB publishable data with human HepG2 cells WB publishable data with human HepG2 cells - SCBT Publication Review
Fecha de publicación: 2015-04-06
Rated 5 de 5 por de Beautiful immunofluorescence cytoplasmic Beautiful immunofluorescence cytoplasmic staining in formalin-fixed HepG2 cells. -SCBT QC
Fecha de publicación: 2013-07-06
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