PTEN Antikörper (A2B1) Empfohlen für die Detektion von PTEN aus der Spezies mouse, rat und human per WB, IP, IF, IHC(P) und ELISA

PTEN Antikörper (A2B1): sc-7974

PTEN Antikörper (A2B1) wurde bewertet mit 4.7 von 5 von 69.
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Datenblatt

    • Anti-PTEN Antikörper A2B1 ist ein monoklonales IgG1 κ PTEN Antikörper, verwendet in 456 wissenschaftlichen Veröffentlichungen, in einer Menge von 200 µg/ml
    • gezogen gegen Aminosäuren 388-400 gelegen am C-terminus von PTEN aus der Spezies human
    • Anti-PTEN Antikörper (A2B1) ist empfohlen für die Detektion von PTEN aus der Spezies mouse, rat und human per WB, IP, IF, IHC(P) und ELISA
    • Anti-PTEN Antikörper (A2B1) ist erhältlich als Konjugat mit Agarose für IP; HRP für WB, IHC(P) und ELISA; und entweder mit Phycoerythrin oder FITC für IF, IHC(P) und FCM
    • auch erhältlich als Konjugat mit Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 oder Alexa Fluor® 647 für IF, IHC(P) und FCM
    • auch erhältlich als Konjugat mit Alexa Fluor® 680 oder Alexa Fluor® 790 für WB (NIR), IF und FCM
    • Bitte kontaktieren Sie uns um ein 10 µg GRATIS Sample vom PTEN (A2B1): sc-7974 zu erhalten.
    • m-IgG Fc BP-HRP (Maus IgG Fc Bindeprotein-HRP) und m-IgGκ BP-HRP (Maus IgGκ Bindeprotein-HRP) sind die empfohlenen Detektionsreagenzien für PTEN Antikörper (A2B1) für WB and IHC(P) Experimente. Diese Reagenzien werden nun auch als Bundle angeboten, zusammen mit PTEN Antikörper (A2B1) (Bestellinformation unten stehend).

Good morning. I used PTEN, sc7974 on prostate tissue. It gave me positive staining in extraglandular tissue and not in glandular structures. Could you guide me for proper steps to obtain immunostaining in the cells of prostatic glands? Thank you

Gefragt von: Nhelal
Thank you for your question. Please contact our Technical Service Department or your local distributor for assistance in troubleshooting your IHC reuslts with this antibody.
Beantwortet von: Tech Service
Veröffentlichungsdatum: 2019-09-26

Good Morning. I want to use PTEN monoclonal for IHC(P) on prostate tissue. I need your advice, Which product is more suitable: sc7974, sc133242, or sc393186? thanks

Gefragt von: Nhelal
Thank you for your question. All three of these PTEN antibodies are recommended for IHC(P) applications. PTEN Antibody (A2B1): sc-7974 is featured in over 300 publications, which you can find right here on the product page.
Beantwortet von: Tech Support Europe
Veröffentlichungsdatum: 2019-09-26

Hallo, when I tried to WB with BT-549(PTEN null as negative control) and Hela(Positive control), I find that non specific bands near 60 kDa in both BT-549 and Hela.  Also, in this datasheet, there are 3 bands in Hela. Which is tha authentic bands?

Gefragt von: taro333
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Beantwortet von: Tech Service
Veröffentlichungsdatum: 2019-02-18

Hello, we are looking for PTEN Antibody to stained our sample which is goat ovary tissue in form of IHC-P. I wish you give me information that PTEN A2B1 (or any of your product) will be reactive to our sample. Thankyou.

Gefragt von: Dimas Reza
Thank you for your question. Unfortunately, we are not aware of any citations using this, or any of our other PTEN antibodies for detection in goat samples. Please contact our Technical Service Department with the protein sequence or accession number for the goat PTEN protein and we would be happy to help you perform sequence analysis to predict any antibodies that may cross-react with your species of interest.
Beantwortet von: Technical Support
Veröffentlichungsdatum: 2018-12-11

WB的稀释比例是多少

Gefragt von: 2333333
感谢您的提问。推荐用于WB的稀释度是1:100-1:1000,关于此问题的详细细节,欢迎通过:电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”,联系我们的技术支持部门。 Thank you for your inquiry. The recommended dilution for WB is 1:100-1:1000. For more detailed information about this question, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website.
Beantwortet von: Technical Support 15
Veröffentlichungsdatum: 2018-02-01

Good afternoon, Would it be possible to get the mentioned free 10 µg Sample of the anti-PTEN antibody PTEN (A2B1): sc-7974 ? Best regards, Ana Lopes

Gefragt von: Ana Lopes
Thank you for your question. Yes, we are happy to provide you with a free 10 µg sample of this antibody. Please call our European Support office toll-free at 00800 4573 8000, email [email protected], or contact us by live chat to request the free sample.
Beantwortet von: Technical Support
Veröffentlichungsdatum: 2017-07-28

I'll soon use Pten antibody for IF of Pten expression in mouse tissues so that I wonder if there's big difference between sc-7974 and sc-133197. Please advise.

Gefragt von: Jessica77
Thank you for your question. The main difference between PTEN (A2B1): sc-7974 and PTEN (B-1): sc-133197 is the immunogen sequence that they were raised against. Both antibodies are recommended for use in mouse tissue by IF. If you require further assistance, please contact Technical Service.
Beantwortet von: Santa Cruz TS
Veröffentlichungsdatum: 2017-05-19

Hi, I am interested in purchasing the PTEN: sc-7974 AC for an IP protocol I am putting together. I was wondering if you could recommend any good controls?

Gefragt von: arwhite
Thank you for your question. This monoclonal PTEN Antibody (A2B1): sc-7974, is available conjugated to agarose (sc-7974 AC) for immunoprecipitation. We would recommend some of the below products as negative and positive controls for this research application: normal mouse IgG-AC: sc-2343 to be used as an negative control MCF7 (IP) Cell Lysate: sc-24793 to be used as a positive control A-431 (IP) Cell Lysate: sc-24781 to be used as a positive control Below is a link to our recommended protocol which also highlights some additional controls and support products available: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Beantwortet von: Technical Support
Veröffentlichungsdatum: 2017-01-27
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Rated 4 von 5 von aus Good detection I detected the bands but they were not very intense. Maybe it was due to the cell type (lung adenocarcinoma)
Veröffentlichungsdatum: 2021-03-17
Rated 5 von 5 von aus Good this antibody works well for western blot with MEF
Veröffentlichungsdatum: 2021-03-10
Rated 5 von 5 von aus good ab Got this ab as a free sample, works very fine on mouse brain lysates. Clear cut band at the right molecular weight, extremely satisfied.
Veröffentlichungsdatum: 2021-02-03
Rated 5 von 5 von aus Great antibody You can get great western blot images by using this antibody.
Veröffentlichungsdatum: 2020-08-13
Rated 5 von 5 von aus good antibody for WB good antibody for WB. PTEN shows up clear at where it should be.
Veröffentlichungsdatum: 2019-09-23
Rated 5 von 5 von aus WB和IP实验结果都很好 我们用了SC-7974和SC-7974(AC),这是我们实验室最常用的抗体之一,SC-7974(AC)功能很强大,可以用于IP实验并避免重链干扰(用Glycin-HCl洗脱),是研究PTEN蛋白修饰和相互作用蛋白的利器。
Veröffentlichungsdatum: 2019-07-02
Rated 5 von 5 von aus Great antibody for WBs I have used this antibody in 1:500 dilution for western blotting using cancer cells and was very impressed with the specificity and quality of signal
Veröffentlichungsdatum: 2019-06-15
Rated 5 von 5 von aus It works great for western blot. sc-7974 works great for western blot with HUVECs cell lines.
Veröffentlichungsdatum: 2018-11-12
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