PFK-1 Antibody (E-4): sc-377346

PFK-1 Antibody (E-4) is a mouse monoclonal IgG3 kappa light chain antibody that detects phosphofructokinase-1 (PFK-1) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-PFK-1 antibody (E-4) is available in a non-conjugated form, allowing for versatile experimental use. PFK-1 plays a crucial role in glycolysis, acting as a regulatory enzyme that catalyzes the conversion of fructose 6-phosphate and ATP into fructose 1,6-bisphosphate, a key step in glucose metabolism. The enzyme is tetrameric and exists in several isoforms, including muscle type (PFKM), liver type (PFKL), and platelet type (PFKP), each adapted to the metabolic needs of different tissues. PFK-1 regulation is vital, as ATP and citrate inhibit enzymatic activity when energy levels are sufficient, while AMP and fructose-2,6-bisphosphate activate PFK-1 during low energy states to promote glycolysis. The interplay between PFK-1 and PFK-2 underscores this enzyme′s importance in maintaining cellular energy homeostasis, making PFK-1 a significant target for studies related to metabolic disorders and energy regulation.

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PFK-1 Antibody (E-4) References:

1. Herpes simplex type 1 activates glycolysis through engagement of the enzyme 6-phosphofructo-1-kinase (PFK-1).  |  Abrantes, JL., et al. 2012. Biochim Biophys Acta. 1822: 1198-206. PMID: 22542512
2. FruBPase II and ADP-PFK1 are involved in the modulation of carbon flow in the metabolism of carbohydrates in Methanosarcina acetivorans.  |  Santiago-Martínez, MG., et al. 2019. Arch Biochem Biophys. 669: 39-49. PMID: 31128085
3. Glycolytic reprogramming in cancer cells: PKM2 dimer predominance induced by pulsatile PFK-1 activity.  |  Shi, X., et al. 2019. Phys Biol. 16: 066007. PMID: 31469100
4. Metformin attenuates hepatoma cell proliferation by decreasing glycolytic flux through the HIF-1α/PFKFB3/PFK1 pathway.  |  Hu, L., et al. 2019. Life Sci. 239: 116966. PMID: 31626790
5. TRAP1 enhances Warburg metabolism through modulation of PFK1 expression/activity and favors resistance to EGFR inhibitors in human colorectal carcinomas.  |  Maddalena, F., et al. 2020. Mol Oncol. 14: 3030-3047. PMID: 33025742
6. SiRNA targeting PFK1 inhibits proliferation and migration and enhances radiosensitivity by suppressing glycolysis in colorectal cancer.  |  Tian, RF., et al. 2020. Am J Transl Res. 12: 4923-4940. PMID: 33042398
7. RNAi-mediated knockdown of PFK1 decreases the invasive capability and metastasis of nasopharyngeal carcinoma cell line, CNE-2.  |  Li, S., et al. 2021. Cell Cycle. 20: 154-165. PMID: 33404290
8. RNAi screening reveals a synthetic chemical-genetic interaction between ATP synthase and PFK1 in cancer cells.  |  Kobayashi, H., et al. 2023. Cancer Sci. 114: 1663-1671. PMID: 36601784
9. Combination of 3PO analog PFK15 and siPFKL efficiently suppresses the migration, colony formation ability, and PFK-1 activity of triple-negative breast cancers by reducing the glycolysis.  |  Kashyap, A., et al. 2023. J Cell Biochem. 124: 1259-1272. PMID: 37450687
10. USP35 promotes breast cancer progression by regulating PFK-1 ubiquitination to mediate glycolysis.  |  Lian, W., et al. 2025. Am J Physiol Cell Physiol. 328: C355-C366. PMID: 39714773

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PFK-1 Antibody (E-4) has 26 citations in a variety of scientific publications.

Primary antibodies like Anti-PFK-1 Antibody (E-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PFK-1 Antibody (E-4) Specifications

Alternate Product Name: PFK-1

Product Clone Name: E-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 681-715

Epitope Target: PFK-1

Epitope Species: human

Product Reactivity/Detection: muscle type PFK-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 26 citations in various scientific publications

Available Conjugates/Forms for PFK-1 Antibody (E-4): P: Blocking Peptide

The preferred PFK-1 antibody is PFK-1 Antibody (G-11): sc-166722.