Parkin Antibody (PRK8): sc-32282
- Parkin Antibody (PRK8) is a mouse monoclonal IgG2b κ Parkin antibody, cited in 319 publications, provided at 200 µg/ml
- raised against recombinant human Parkin
- Parkin Antibody (PRK8) is recommended for detection of Parkin of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
- Anti-Parkin Antibody (PRK8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Parkin Antibody (PRK8) for WB and IHC(P) applications. These reagents are now offered in bundles with Parkin Antibody (PRK8) (see ordering information below).
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Parkin Antibody (PRK8) is a mouse monoclonal IgG2b kappa light chain antibody that targets Parkin, also known as PRKN, PARK2, or parkin RBR E3 ubiquitin protein ligase, with confirmed reactivity in human, mouse, and rat samples. Parkin is a 465-amino acid E3 ubiquitin ligase encoded by the PARK2 gene, essential for the ubiquitination process, which tags proteins for degradation, maintaining cellular homeostasis by eliminating damaged or misfolded proteins. Parkin is particularly significant in mitophagy, the selective autophagic removal of dysfunctional mitochondria, which is crucial for preserving mitochondrial quality and function. Mutations in the PARK2 gene are associated with autosomal recessive juvenile Parkinson′s disease, highlighting Parkin′s importance in neuronal health. Anti-Parkin antibody (PRK8) is raised against recombinant human Parkin, targeting an epitope within the protein′s C-terminal region. Parkin (PRK8) monoclonal antibody is validated for diverse applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Parkin expression is observed in neurons across various brain regions, including the basal ganglia, midbrain, cerebellum, and cerebral cortex, with roles that are evolutionarily conserved across species. Anti-Parkin antibody (PRK8) serves as a critical tool for studying Parkin′s functions and involvement in cellular processes and neurodegenerative research.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Parkin Antibody (PRK8) References:
- Differential expression of the parkin gene in the human brain and peripheral leukocytes. | Sunada, Y., et al. 1998. Neurosci Lett. 254: 180-2. PMID: 10214987
- Parkin suppresses unfolded protein stress-induced cell death through its E3 ubiquitin-protein ligase activity. | Imai, Y., et al. 2000. J Biol Chem. 275: 35661-4. PMID: 10973942
- Parkin is associated with actin filaments in neuronal and nonneural cells. | Huynh, DP., et al. 2000. Ann Neurol. 48: 737-44. PMID: 11079537
- Parkin expression in the adult mouse brain. | Stichel, CC., et al. 2000. Eur J Neurosci. 12: 4181-94. PMID: 11122330
- Immunodetection of Parkin protein in vertebrate and invertebrate brains: a comparative study using specific antibodies. | Horowitz, JM., et al. 2001. J Chem Neuroanat. 21: 75-93. PMID: 11173222
- Parkin and Parkinson's disease. | Mizuno, Y., et al. 2001. Curr Opin Neurol. 14: 477-82. PMID: 11470964
- E3 ubiquitin-protein ligase activity of Parkin is dependent on cooperative interaction of RING finger (TRIAD) elements. | Rankin, CA., et al. 2001. J Biomed Sci. 8: 421-9. PMID: 11549885
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Parkin Antibody (PRK8) | sc-32282 | 200 µg/ml | $322.00 | |||
Parkin Antibody (PRK8): m-IgG Fc BP-HRP Bundle | sc-528453 | 200 µg Ab; 10 µg BP | $361.00 | |||
Parkin Antibody (PRK8): m-IgGκ BP-HRP Bundle | sc-520834 | 200 µg Ab, 40 µg BP | $361.00 | |||
Parkin Antibody (PRK8): m-IgG2b BP-HRP Bundle | sc-550038 | 200 µg Ab; 10 µg BP | $361.00 | |||
Parkin Antibody (PRK8) AC | sc-32282 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Parkin Antibody (PRK8) HRP | sc-32282 HRP | 200 µg/ml | $322.00 | |||
Parkin Antibody (PRK8) FITC | sc-32282 FITC | 200 µg/ml | $336.00 | |||
Parkin Antibody (PRK8) PE | sc-32282 PE | 200 µg/ml | $349.00 | |||
Parkin Antibody (PRK8) Alexa Fluor® 488 | sc-32282 AF488 | 200 µg/ml | $364.00 | |||
Parkin Antibody (PRK8) Alexa Fluor® 546 | sc-32282 AF546 | 200 µg/ml | $364.00 | |||
Parkin Antibody (PRK8) Alexa Fluor® 594 | sc-32282 AF594 | 200 µg/ml | $364.00 | |||
Parkin Antibody (PRK8) Alexa Fluor® 647 | sc-32282 AF647 | 200 µg/ml | $364.00 | |||
Parkin Antibody (PRK8) Alexa Fluor® 680 | sc-32282 AF680 | 200 µg/ml | $364.00 | |||
Parkin Antibody (PRK8) Alexa Fluor® 790 | sc-32282 AF790 | 200 µg/ml | $364.00 |